应用DASS-FAT诊断华支睾吸虫病  

Study on using DASS-FAT to detect clonorchiasis sinensis

作　　者：徐凤全[1] 时法茂[1] 张志华[1] 魏庆宽[1] 孔庆安[1] 杨艳君[1] 

机构地区：[1]山东省寄生虫病防治研究所,济宁272033

出　　处：《中国公共卫生》2003年第4期420-421,共2页Chinese Journal of Public Health

摘　　要：目的　探讨DASS -FAT方法检测血清抗体诊断华支睾吸虫病的应用价值。方法　通过人工培养华支睾吸虫成虫获取ES抗原。将ES抗原与Sepharose - 4B偶联 ,应用FAT技术测定血清中华支睾吸虫特异性抗体。 结果　 83例华支睾吸虫感染者血清全部呈阳性反应 ,阳性率为 10 0 % ,健康人群的抗体阳性率为 2 0 4 % (2 8/ 1372 ) ;88例脑囊虫病及 5 5例肠道寄生虫感染者血清的抗体阳性率分别为 2 2 7% (2 / 88)和 1 80 % (1/ 5 5 ) ,与健康人群比较无明显交叉阳性反应 (χ2 =0 0 2 1,P >0 1) ;华支睾吸虫感染者经治疗后 12个月 ,血清特异抗体检出率明显下降 ,有77 78% (2 8/ 36 )的感染者抗体转阴。结论　应用DASS -FST方法检测血清中华支睾吸虫特异性抗体具有较高的敏感性和特异性 ,非华支睾吸虫感染者血清无明显交叉阳性反应。该方法简便、快速、稳定性好 ,可用于华支睾吸虫病的感染诊断、疗效评价、流行病学调查及基础免疫研究。Objective To search the use value of DASS-FAT in diagnosis of infection with clonorchiasis sinensis.Methods By the external culture of clonorchiasis sinensis to obtain the ES antigen and join it to Sepharous-4B,using FAT to detect sera antigen of the patients with clonorchiasis sinensis.Results The special antibody positive rate was 100% in sera of the patients with clonorchiasis sinensis.In the helath people,the cerebral cysticercosis,and the infection with intestinal worm,the positive rate was 2.04%(28/ 1 372 ),2.27%(2/88),and 1.80%(1/55).The cross-reactive was nonsignificant(χ 2=0 021, P >0 1).The negative rate was 77.78%(28/36) of the antibody in sera with clonorchiasis sinensis after treatment (12 months).Conclusion Using DASS-FAT to detect the special antibody in sera of the patients with clonorchiasis sinensis had high specificity and sensitivity,and the cross-reactive was nonsignificant in uninfected with clonorchiasis sinensis.The method was simple,rapid and the result of the test was stable.It can be used in the diagnosis,the examination of the effect of treatment,epidemiology and the basic study in clonorchiasis sinensis.

关 键 词：DASS-FAT 华支睾吸虫病 限定抗原底物珠系统 荧光抗体试验 琼脂糖珠-4B 

分 类 号：R532.23[医药卫生—内科学]