乌头碱对bcl-2基因表达影响作用的定量研究  

Quantitative study on the effect of aconitine on the expression of bcl-2 gene

作　　者：饶朝龙[1] 彭成[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2015年第4期14-16,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(2012CB723502);四川省科技厅重点项目(2012JYZ006);四川省科技厅"中药药理四川省青年基金科技创新研究团队"(2014TD0007);四川省教育厅重点项目(12ZA044)

摘　　要：目的:研究乌头类中药对bcl-2基因表达的影响,探讨乌头类中药毒性作用机制。方法:建立荧光定量PCR反应体系;体外培养Hep G2细胞,分别以500,100,50和10μg.m L-1浓度的乌头碱作用于细胞,37℃染毒1.5 h,荧光定量PCR仪检测。结果:乌头碱作用下,bcl-2基因表达量增加,且不同剂量组呈现剂量-反应关系。结论:乌头类中药具有促进bcl-2基因表达的作用,可能是其毒效作用的重要因素。Objective: To study the effect of aconitic alkaloid on the expression of bcl-2 gene, and explore the mechanism of toxicity. Method: The reaction system of Q-RT-PCR was established. Hep G2 cell was cultivated in vitro, then treated with aconitine(500,100,50 and 10 μg.m L-1) respectively for 1. 5 h at 37℃. And then the cell was determined with Q-RT-PCR. Result: After treated by aconitine, the amount of expression of bcl-2 gene was increased significantly. And its dose-response relationship was observed. Conclusion: Aconitic alkaloid plays an important role in promoting bcl-2 gene expression which may be the mechanism of aconitic toxicity.

关 键 词：乌头碱 BCL-2基因 毒性 定量 

分 类 号：R285[医药卫生—中药学]