人类睾丸生精细胞凋亡相关基因TSARG3的克隆  

Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells

作　　者：刘刚[1] 卢光琇[1] 傅俊江[1] 刘上峰[1] 邢晓为[1] 李麓芸[1] 

机构地区：[1]中南大学湘雅医学院人类生殖工程研究室,长沙410078

出　　处：《中华医学遗传学杂志》2003年第2期107-110,共4页Chinese Journal of Medical Genetics

基　　金：973项目 (GI9990 5590 1 )

摘　　要：目的　克隆人类睾丸生精细胞凋亡相关基因TSARG3。方法　从已获得的小鼠隐睾和正常睾丸对照中表达量有明显差异的表达序列标签片段 (BE64 45 3 7)入手 ,构建人同源表达序列标签重叠群 ,应用基因特异性引物和载体特异性引物 ,在睾丸cDNA文库的DNA中进行巢式PCR扩增、测序 ,对测序结果进行生物信息学分析。结果　从睾丸cDNA文库中分离出人类睾丸凋亡相关基因的 5′末端而获得全长cD NA ,命名为TSARG3 ,GenBank登录号为AF4192 91(保密期为 1年 ) ,同时应用相同方法克隆了该基因在小鼠中的同源基因 ,GenBank登录号为AF4192 92。结论　获得人类睾丸生精细胞凋亡相关基因TSARG3 ,该基因可能与人类睾丸生精细胞凋亡有关。Objective: To clone a novel gene which is related to human testis spermatogenesis apoptosis. Methods: To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library, the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed. Results: The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method. Conclusion: A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.

关 键 词：基因克隆 巢式聚合酶链反应 睾丸 精子 细胞凋亡 男性不育 

分 类 号：R346[医药卫生—基础医学]