前列腺素E_1对输尿管梗阻大鼠肾间质纤维化及转化生长因子β_1 mRNA表达的影响  

The Antagonistic Effect of PGE_1 on TGFβ_1 mRNA Expression and Rat Renal Interstitial Fibrosis Induced by Unilateral Ureleral Obstruction

作　　者：郭虹[1] 朱妙珍[2] 段建[1] 曹礼应[1] 

机构地区：[1]成都军区昆明总医院肾脏科,云南昆明650032 [2]第三军医大学大坪医院肾科

出　　处：《中国医师杂志》2003年第3期359-361,377,共4页Journal of Chinese Physician

摘　　要：目的　探讨前列腺素E1(PGE1)拮抗肾间质纤维化的作用。方法　建立单侧输尿管梗阻 (UUO)大鼠肾小管间质病变模型 ,应用原位杂交、病理形态学观察、生化分析等技术 ,研究PGE1用药后肾组织中转化生长因子 β1(TGFβ1)mRNA的表达 ,肾小管间质病变及羟脯氨酸含量的变化。结果　大鼠UUO术后 3d ,肾间质水肿、单个核细胞浸润 ;14d后肾小管萎缩 ,间质纤维增生 ,间质面积增宽 ,伴随肾皮质中羟脯氨酸含量升高及TGFβ1mRNA表达增多。后者的峰值出现先于羟脯氨酸峰值及间质面积增宽。PGE1可下调TGFβ1mRNA表达及羟脯氨酸含量 ,抑制间质中单个核细胞浸润。结论　PGE1改善UUO大鼠肾间质纤维化的原因至少与抑制单个核细胞浸润及TGFβ1mRNA表达有关。Objective To explore the possible role of PGE 1 in tubulointerstitial fibrosis formation. Methods Rat model of unilateral ureteral obstruction(UUO) was established.In situ hybridation(ISH) technique, morphological observation and biochemical analysis were applied to determine TGFβ 1 mRNA expression, changes of tubulointerstitium and rencortical hydroxyproline content after used PGE 1. Results 3 days after UUO,the rats renal interstitial edema, a number of mononuclear cells recruitment. On day 14,tubule atrophy, interstitial proliferative fibrosis and area enlarged,hydroxyproline content,TGFβ 1 mRNA expression increased, the peak value of the latter occurred early than that of hydroxyproline peak value and interstitial area enlargement.PGE 1 could down -regulate these changes.Conclusions The antagenistic effect of PGE 1 on renal interstitial fibrosis may be partly associated with down-regulating overexpression of TGFβ 1 mRNA and inhibiting mononuclear cells recruitment.

关 键 词：输尿管梗阻 前列腺素E1 转化生长因子β1 小管间质纤维化 

分 类 号：R693[医药卫生—泌尿科学]