油菜种子蛋白质溶出率及蛋白质组分分析  

Analysis of solubility and ingredients of rapeseed protein

作　　者：何应森[1] 刘尚旭[2] 文建军[3] 

机构地区：[1]四川教育学院生化系,四川成都610041 [2]重庆教育学院生物系,重庆400067 [3]四川大学生命科学院,四川成都610064

出　　处：《西南农业学报》2003年第1期45-47,共3页Southwest China Journal of Agricultural Sciences

摘　　要：研究了油菜种子蛋白质的溶出条件 ,发现选用Na2 HPO4-NaOH ,pH11 4缓冲液 ,蛋白质溶出率达 94%以上。用 80 %甲醇脱色 ,可以将油菜种子中大部分干扰物质脱去 ,要达到完全去掉干扰物质则以反抽法最佳 ,且得到的一些组份溶解性良好。The extracting conditions of rapeseed protein were studied.It was found that when Na 2HPO 4-NaOH buffer(pH=11.4) was used,the dissoluble ratio of rapeseed protein was over 94?%.Most of interfering materials could be excluded with 80?% methanol as decoloring agent.The countercurrent extraction was the best way to exclude the interfering materials and some of these ingredients extracted were well dissolved.

关 键 词：甘蓝型油菜籽 种子贮藏蛋白 反抽法 柱层析 

分 类 号：S312[农业科学—作物栽培与耕作技术] S565.4[农业科学—农艺学]