肝炎患者中TT病毒DNA及其IgG抗体的检测  

Detection of TTV DNA and Anti-TTV IgG in Sera from Hepatitis Patients

作　　者：徐冉行[1] 凌志强[2] 张勤[3] 

机构地区：[1]浙江省宁波市第六医院检验科,315040 [2]浙江省医学科学院,310013 [3]浙江省杭州市第六人民医院,310014

出　　处：《中国误诊学杂志》2003年第1期4-6,共3页Chinese Journal of Misdiagnostics

摘　　要：目的　了解肝炎患者中 TTV的感染情况。方法　应用 Nested- PCR对 137份甲～庚型肝炎、31份非甲～庚型肝炎及 10 4份献血员进行 TTV DNA检测 ,同时用 EL ISA法检测抗 TTVIg G。结果　 TTV基因检出率分别为甲～庚型肝炎 2 0 .4 4 %、非甲～庚型肝炎 2 9.0 3% ,献血员 13.4 6 % ;抗 TTV Ig G检出率分别为甲～庚型肝炎 2 7.74 %、非甲～庚型肝炎 35 .4 8% ,献血员 14 .4 2 % ;甲～庚型肝炎、非甲～庚型肝炎与献血组相比 TTV DNA及抗 TTV Ig G均存在显著性差异 (P<0 .0 5 )。结论　肝病中存在 TTV感染 ,TTV存在健康携带状态。Objective To investigate the prevalence of TT virus (TTV) in hepatitis patients.Methods TTV DNA and anti TTV IgG were simultaneously tested by nested PCR method using primers from ORF1 of TTV genome DNA and ELISA ,respectively, in 137 hepatitis A E patients,31 non A E patients and 104 healthy blood donors.Results The positive rates of TTV DNA were as follows: 20.44%(28/137) in A E hepatitis patients; 29.03%(9/31) in non A E hepatitis patients; 13.46%(14/104) in blood donors. There were significant difference between every two groups, respectively (P<0.05). The positive rates of anti TTV IgG were 27.74%(38/137) in A E hepatitis patients; 35.48%(11/31) in non AE hepatitis patients and 14.42%(15/104) in blood donors. There were also significant difference between every two groups, respectively (P<0.05).Conclusion Our investigation suggested that there existed TTV infection among hepatitis patients. Some of the normal and blood donor groups infected with TTV could become healthy carries of the virus. TTV might be transmitted by blood transfusion. The anti TTV IgG might be used as maker for detection of TTV infection.

关 键 词：病毒性肝炎 输血传播病毒 聚合酶链反应 免疫球蛋白G 带病原状态 

分 类 号：R512.6[医药卫生—内科学]