芪丹通脉片对大鼠急性心肌缺血损伤的影响  

Effects of Qidantongmai tablet on acute myocardial ischemia injury in rats

作　　者：行利[1] 马静[1] 王宗仁[1] 杨喜忠[2] 马世平[1] 李萌萌[3] 肖铁卉[1] 李晶华[1] 郑谨[1] 

机构地区：[1]第四军医大学西京院中医科,陕西西安710033 [2]第四军医大学西京院药剂科,陕西西安710033 [3]第四军医大学西京院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2003年第5期415-417,共3页Journal of the Fourth Military Medical University

基　　金：陕西省重点科研项目资助课题 (2 0 0 1K1 2 G7)

摘　　要：目的 :观测芪丹通脉片 (QDTMT)急性心肌缺血损伤的影响 .方法 :用SD大鼠皮下注射异丙肾上腺素 (Iso,5mg·kg-1)诱导心肌缺血模型 ,观测心肌线粒体 (Mit)三磷酸腺苷酶(ATPase)活力、Ca2 + 含量 ;在电镜下 ,观察心肌线粒体超微结构的变化 .结果 :QDTMT组Ca2 + ATPase ,Mg2 + ATPase及Ca2 + Mg2 + ATPase活性显著高于模型组 [(4 0 .9± 8.1)vs(2 3.1± 5 .7) ,(4 0 .4± 4 .4 )vs(2 1.7± 6 .6 ) ,(94 .7± 8.6 )vs (6 3.5± 9.5 )mmol·g-1,P <0 .0 5 ];QDTMT组与模型组相比Ca2 + 显著降低 [(7.6± 1.6 )vs(12 .9± 1.3) ,(37.3± 5 .3)vs(73.2± 8.9)mmol·mg-1,P <0 .0 1].同时 ,与对照组相比 ,心肌超微结构的损害明显减轻 .结论 :QDTMT减轻钙超载和保护心肌细胞超微结构的作用 ,可能是其改善心肌缺血的作用机制之一 .AIM: To study the effects of Qidantongmai tablet (QDTMT) on acute myocardial ischemia in rats. METHODS: Acute myocardial ischemia was induced by subcutaneous injection of isoproterenol (ISP. 5 mg·kg -1 ) into SD rats. Activities of ATPase and content of Ca 2+ were observed in myocardial tissue and mitochondria. The changes of myocardial ultrastructure were examined by transmission electron microscope. RESULTS: Compared with those of the controls, activities of ATPase in mitochondrion were strengthened in QDTMT group [(40.9±8.1) vs (23.1± 5.7), (40.4±4.4) vs (21.7±6.6),(94.7±8.6) vs (63.5± 9.5) mmol·g -1 , P <0.05] and the content of Ca 2+ in mitochondrion was decreased [(7.61±1.62) vs (12.94±1.28),(37.3±5.3) vs (73.2±8.9) mmol·mg -1 , P <0.01] while the ultrastructure of myocardial damage was significantly reduced in QDTMT group ( P <0.01). CONCLUSION: QDTMT can reliecve calcium overload in cardiac mitochondrion and protect the integrity of the ultrastructure of cardiac mitochondrion, which may be in part responsible for the mechanism of QDTMT in ameliorating ischemia.

关 键 词：芪丹通脉片 大鼠 急性心肌缺血损伤 影响 中药 中医药疗法 

分 类 号：R259.422[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]