当归补血汤血清抑制人肝癌细胞SMMC-7721的增殖作用  

DangGuiBuXue Decoction in inhibiting proliferation of human liver cancer SMMC-7721 cells in vitro

作　　者：李军昌[1] 赵建斌[1] 刘颖格[2] 成晓娟[1] 马世平[1] 王文[1] 郑谨[1] 夏天[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710033 [2]第四军医大学西京医院呼吸内科,陕西西安710033

出　　处：《第四军医大学学报》2003年第5期436-437,共2页Journal of the Fourth Military Medical University

摘　　要：目的 :观察当归补血汤血清对SMMC 772 1增殖影响的量效和时效关系 .方法 :采用纯种新西兰雄兔 2 5只 ,随机分 5组 ,分别给予 5 0mL的 32 ,16 ,8和 4g·kg-1当归补血汤和生理盐水灌胃 ,于不同时间点收集血清 ,以MTT法测定上述血清对人肝癌细胞系SMMC 772 1增殖抑制作用 .结果 :当归补血汤血清对人肝癌细胞SMMC 772 1增殖有抑制作用 ,且具有时间和剂量依赖性 ,灌胃后 4 5min抑制作用最强 ,各组抑制强度分别为 0 .4 6± 0 .0 5 (32g·kg-1,P <0 .0 1) ,0 .5 3± 0 .0 6(16g·kg-1,P <0 .0 1) ,0 .5 5± 0 .0 4 (8g·kg-1,P <0 .0 5 ) ,0 .6 1± 0 .0 3(4g·kg-1,P >0 .0 5 ) .ED5 0为 12 2g·kg-1;效应半衰期为 2 .2 0h .结论 :当归补血汤经肠道进入体内具有一定的抑制肝癌细胞增殖的作用 。AIM: To observe the effects of the serum of DangGuiBuXue Decoction (DGBXD) on the proliferation of SMMC 7721. METHODS: 25 rabbits were divided into 5 groups at random and the rabbits sera of different time points were collected after ig 50 mL of Naltrii chlorcid (9 g·L -1 ) or DGBXD with the dosage of 32 g, 16, 8, 4 g·kg -1 . The culture medium of human heptocarinoma cell lines of SMMC 7721 was treated by the rabbits sera. Effects of the sera on inhabiting proliferation of SMMC 7721 were detected by MTT assay. RESULTS: The sera of DGBXD ig inhibited the proliferation of SMMC 7721 dose and time dependent manner. Tmax was 45min after ig and the maximum effects of BGBXD was 0.46±0.05 (32 g·kg -1 , P <0.01), 0.53±0.06 (16 g·kg -1 , P <0.01), 0.55±0.04 (8 g·kg -1 , P <0.05), 0.61±0.03 (4 g·kg -1 , P >0.05) vs control. ED50 was 122 g·kg -1 and T 1/2 was 2.2 h. CONCLUSION: The sera of DangGuiBuXue Decoction can inhibit the proliferation of human liver cancer SMMC 7721 in a dose and time dependent manner.

关 键 词：肝肿瘤 当归补血汤 血汤 MTT法 中医药疗法 

分 类 号：R273.57[医药卫生—中西医结合]