扶正抑瘤饮抑瘤作用的实验研究  

Study on the inhibitive effects of a traditional Chinese preparation-Fu-Zheng-Yi-Liu-Yin on cancer

作　　者：邱根全[1] 刘昳[1] 王锐[1] 南克俊[2] 

机构地区：[1]西安交通大学第一医院中医科,陕西西安710061 [2]西安交通大学第一医院肿瘤科,陕西西安710061

出　　处：《第四军医大学学报》2003年第5期466-468,共3页Journal of the Fourth Military Medical University

基　　金：陕西省中医药管理局 (0 4 )

摘　　要：目的 :观察扶正抑瘤饮对人胃癌细胞株体外增殖的影响 ,及其对小鼠移植性肉瘤的抑制作用 .方法 :应用MTT比色法 ,检测不同浓度扶正抑瘤饮 (简称中药ZY)对不同分化程度的 3株人胃癌细胞增殖的影响 .并以小鼠移植性肉瘤180为模型 ,随机分空白对照组 ,ELF化疗方案组 (ELF) ,扶正抑瘤饮组 (ZY) .观察其对小鼠移植性肉瘤的抑制作用 .结果 :中药对 3株胃癌细胞均呈现一定程度的抑制作用 ,但其对不同细胞株敏感性有差异 .中药浓度在 1× 10 -2 ～ 1× 10 3mg·L-1之间 ,对SGC 790 1,880 1细胞株增殖的抑制作用与剂量无明显相关 ,而对MGC 80 3细胞株增殖的抑制作用随浓度升高而增强 ,当浓度为 1× 10 3 mg·L-1时 ,抑制率最大 (4 1.1±5 .4 ) % .中药对小鼠移植性肉瘤 (91.0 % )与ELF组肿瘤生长抑制率 (93.1% ) ,ZY组平均瘤重 (0 .131± 0 .0 18)g与ELF组(0 .0 10± 0 .0 0 8)g相比 ,均无显著性差异 (P >0 .0 5 ) .ZY组小鼠体质量变化量 (6 .80± 0 .5 4 )g与空白对照组 (5 .5 0± 2 .2 7)g相比无显著性差异 (P >0 .0 5 ) ,但ELF组小鼠体质量变化量(0 .4 3± 0 .15 )g明显小于空白对照组和ZY组 (P <0 .0 5 ) .结论 :扶正抑瘤饮对人胃癌细胞株SGC 790 1,880 1的抑瘤作用与浓度无明显关系 ,但对MGC 80 3细胞株的抑?AIM: To evaluate the inhibiting effects of traditional Chinese medicine Fu Zheng Yi Liu Yin (ZY) on gastric cancer cell lines in vitro and on mice transplanted sarcoma 180 in vivo. METHODS: MTT was used to measure the levels of the proliferation of various differentiated gastric carcinoma cell lines cultured with ZY in different concentrations. Mice transplanted sarcoma 180 was used as a model. Mice was randomly divided into three groups as follows: control group, ELF (Etoposide Leucovorin Five fluorouracil) group and ZY group. Mice survival rate and the change of weight were observed. RESULTS: The proliferation of MGC 803 cell lines was obviously inhibited by ZY in a dose dependent manner and the inhibitory rate came to the peak at (41.1±5.4)%, when cultured with ZY at concentration of 103 mg·L -1 , while the inhibitory rate of 8801 and SGC 7901 cell lines didn't increase with the increasing of the concentration of ZY. The inhibitive ratio of the growth of mice transplanted sarcoma by ELF therapy was 93.1% and that by ZY groups was 91%, both of which were much higher than that in the control group. The tumor weight of ELF group (0.010±0.008) g was lower than that of ZY group (0.131±0.018) g, and there was no significant difference between them, but both of them were much lower than that of control group (1.45±0.30) g. Significant weight reducing was observed in ELF group(0.43±0.15)g while the weight of ZY group(6.80±0.54)g was similar to that of the control group(5.50±2.27)g. CONCLUSION: The inhibitory rate of 8801 and SGC 7901 cell lines is not related with the concentration of ZY, while that of MGC 803 cell lines increases with the concentration of ZY. ZY has the similar inhibitive ratio as that of ELF in mice transplanted sarcoma 180, but it has no effects on the weight of the mice.

关 键 词：扶正抑瘤饮 抑瘤作用 实验研究 中药 药理 

分 类 号：R285.5[医药卫生—中药学]