转化生长因子β_1及其受体在主动型海曼肾炎发生中的作用  

The role of transforming growth factor-β_1 and its receptor in the pathogenesis of active Heymann nephritis

作　　者：杨幼萍 王照明[2] 张建民[2] 朱杨丽 林曦 

机构地区：[1]温岭市第一人民医院病理科,317500 [2]东南大学医学院附属中大医院病理科

出　　处：《浙江医学》2003年第3期143-145,共3页Zhejiang Medical Journal

摘　　要：目的 研究转化生长因子 β 1(TGF - β 1)及其受体TβRⅠ在不同病变阶段主动型海曼肾炎 (AHN)大鼠肾组织内的表达情况 ,进而探讨TGF - β 1 在AHN病变发生及发展中的作用。 方法 提取SD大鼠肾近曲小管的抗原FxlA ,建立免疫SD大鼠AHN ,并分为实验组和对照组。第4、6、8及12周分别处死实验组及对照组大鼠 ,用免疫组化SP法检测TGF- β 1、TβRⅠ在不同病变阶段AHN大鼠肾组织内的表达情况 ,用计算机图像分析系统比较上述成分在实验组及对照组大鼠肾组织内的相对含量。 结果 自AHN诱发后第4周开始 ,随大鼠尿蛋白的升高 ,TGF- β 1、TβRI在大鼠肾组织内的表达即开始上调 ,至第8周达最高峰 ,第12周有所下调。定量测得TGF -β 1、TβRⅠ在AHN大鼠肾小球内的表达明显高于对照组 (P<0.05)。结论 TGF- β 1、TβRI在不同病变阶段AHN大鼠肾组织内的表达均上调。TGF- β1 可能通过刺激肾小球上皮细胞分泌过量的细胞外基质 (ECM) ,使AHN大鼠肾小球毛细血管基底膜内ECM的含量增加 ,提示TGF- β 1 在AHN病变的发生及发展中起着重要作用。Objective To investigate the expression of transforming growth factor-β 1 (TGF-β 1 )and its receptor(TβRⅠ)in the renal tissue of rats with active Heymann nephritis(AHN)and to explore their role in the pathogenesis of AHN.Methods SD rats were immunized with FxlA to induce AHN.AHN and control rats were sacrificed at4?6?8and12weeks.The expression of TGF-β 1 and TβR I in rat renal tissue were detected with SP immunohistochemical technique and were analyzed semi-quantitatively with the computer imaging analysis system.Results At the4th week after the induction of AHN,the expression of TGF-β 1 and TβRⅠin the renal tissue of AHN rats were up-regulated.At the8th week the expression of TGF-β 1 and TβRⅠreached a peak and at the12th week these two factors slightly declined(P<0.05).Conclusions The expresˉsion of TGF-β 1 and TβRⅠwere up-regulated in the kidneys of the rats with AHN and TGF-β 1 may play an important role in the pathogenesis of AHN.

关 键 词：海曼肾炎 转化生长因子Β1 细胞外基质 转化生长因子Β1受体 

分 类 号：R692[医药卫生—泌尿科学]