复合壳多糖人工皮肤移植真皮再构建的初步研究  

Primary study on dermal reconstruction of composite chitosan artificial skin after grafted onto experimental rabbit

作　　者：李静[1] 伍津津[1] 鲁元刚[1] 朱堂友[1] 李文维[1] 贺萍[1] 杨宏珍[1] 

机构地区：[1]解放军第三军医大学大坪医院野战外科研究所皮肤科,重庆市4000420

出　　处：《中国临床康复》2003年第4期572-573,T002,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39870238)

摘　　要：目的:探讨复合壳多糖人工皮肤移植实验家兔后血管和基底膜的形成情况,以了解移植后真皮的再构建情况。方法:别将复合壳多糖人工皮肤移植前、移植后1个月、移植后2个月、正常人皮肤、正常兔皮肤石蜡块切片,用抗第Ⅷ因子多抗及抗层粘连蛋白多抗进行免疫组化染色,观察血管和基底膜的形成情况。结果:合壳多糖人工皮肤移植后1个月及2个月真皮内可见较多新生血管,以移植后1个月明显,且真皮浅层血管数量较正常皮肤多;部分表、真皮交界处层粘连蛋白抗体免疫组化染色阳性,可见不连续的基底膜。结论:复合壳多糖人工皮肤移植实验家兔后真皮再构建良好,是理想的组织工程皮肤材料。AIM:To study the reformation of blood vessel and basement membrane after compo site chitosan artificial skin grafted onto experimental rabbit in order to under stand the dermal reconstruction of composite chitosan artificial skin and to pro vide experimental evidences for its clinical use.METHODS:By immunohistochemical staining,paraffin sections of composite chitosan artificial skin before grafted, one month or two months after grafted,natural human skin and natural rabbit ski n were stained with polyclonal antibodies to factor Ⅷ related antigen and lami nin respectively to show the reformation of blood vessles and basement membrane. RESULT:Many neonate blood vessles were observed in the dermis,which were special y obvious in one month after grafted.The quantities of blood vessles in the derm is were more than natural dermis.Discontinous basement membrane at the junction of epidermis and dermis of one month or two months after grafted was observed.CO NCLUSION:There are obvious neonate blood vessles in the dermis,indicating compos ite chitosan artificial skin can achieve reconstruction of blood vessles, the reformation of basement membrane is partial and discontinous. The dermal reconst ruction of composite chitosan artificial skin after grafted was well.Composite c hitosan matrix is an ideal material for tissue engineering skin.

关 键 词：复合壳多糖 人工皮肤移植 真皮再构建 

分 类 号：R622.1[医药卫生—整形外科]