D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族的遗传多态性  

Sequence Analysis and Genetic Polymorphisms of D4S1647 and D6S2414 Loci in Chinese Han,Mongolian and Tibetan Populations

作　　者：汤智育[1] 廖淼[1] 胡羽[1] 梁伟波[1] 张林[1] 陈国弟[1] 

机构地区：[1]四川大学华西基础医学与法医学院物证学教研室,成都610041

出　　处：《四川大学学报（医学版）》2003年第2期223-226,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 0 1710 3 3 )资助

摘　　要：目的　调查 D4 S16 4 7、D6 S2 4 14基因座在中国汉族、蒙古族、藏族群体中的遗传分布规律。方法　采集 30 8份血及唾液标本应用 PCR技术 ,扩增产物用非变性聚丙酰胺凝胶电泳分离 ,银染显色分析。结果　两位点各群体基因型频率分布均符合 Hardy- Weinberg平衡 ,每一位点等位基因频率分布在各群体间均无显著差异 ;通过对 10个汉族家系的遗传模式分析 ,证实了两位点等位基因传递遵循孟德尔遗传规律。结论　 D4 S16 4 7、D6 S2 4 14基因座在中国汉族、蒙古族。Objective To investigate the polymorphisms of two short tandem repeats (STR) loci D4S1647 and D6S2414 in Chinese Han,Mongolian and Tibetan populations. Methods Blood and saliva samples were collected from 308 unrelated individuals in the three populations. DNA was extracted by chelex 100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed by nondenaturing polyacrylamide gel electrophoresis and silver staining. Results The genotype distributions of D4S1647 and D6S2414 loci were in accordance with Hardy Weinberg equilibrium.The frequencies of alleles showed no significant differences among the three populations. Analyses of Han families indicated that the alleles agreed with Mendelian inheritance law. Conclusion This study yielded information on the genetic polymorphisms of the two STR loci in Chinese Han,Mongolian and Tibetan populations.

关 键 词：短串联重复序列 聚合酶链反应 D4S1647、D6S2414基因库 遗传多态性 

分 类 号：Q987[生物学—遗传学]