HPLC法同时测定布洛伪麻分散片中布洛芬盐酸伪麻黄碱的含量  

HPLC for the simultaneous determination of Ibuprofen and Pseudoephedrine hydrochloride in Ibuprofen and Pseudoephedrine dispersible tablets

作　　者：张文军[1] 焦建杰[1] 肖向茜[1] 董伟林[2] 张才丽[1] 娄建石[1] 

机构地区：[1]天津医科大学药理学教研室 [2]天津医科大学实验中心,天津300070

出　　处：《天津医科大学学报》2003年第1期21-23,共3页Journal of Tianjin Medical University

摘　　要：目的 :建立同时测定布洛伪麻分散片中布洛芬和盐酸伪麻黄碱的含量的反相HPLC测定方法。方法 :色谱柱为YWG -C18,流动相 :甲醇 -0.01mol/L磷酸二氢钾 -磷酸 (75:25:0.1pH=3.6)、检测波长 :210nm ,流速 :1.0ml/min;进样量20μl。结果 :布洛芬和盐酸伪麻黄碱分别在20.0～400.0μg/ml;3.0～60.0μg/ml的范围内浓度与峰面积线性关系良好 ,相关系数分别为r=1.0000和r=0.9999。两组分的回收率分别介于100.67 %～101.40 %和99.66%～101.64 %之间 ,日内和日间RSD均低于5%。结论 :可同时测定布洛伪麻分散片中布洛芬和盐酸伪麻黄碱的含量 ,而且简便、准确、快速 ,可用于批次样品的含量测定及质量控制。Objective:To establish a reversed-phase and exstandard HPLC method for the simultaneous determination of Ibuprofen and Pseudoephedrine hydrochloride in Ibuprofen and Pseudoephedrine dispersible tablets.Methods:The two contents were assayed on YWG-C 18 column with a mobile phase com-posed of methonal-phosphate buffer solution(0.01mol/L KH 2 PO 4 and phosphate)(75:25:0.1pH=3.6)at a flow rate1.0ml/min.The dedectable wavelength was210nm.Results:The linear ranges of Ibuprofen and Pseudoephedrine hyˉdrochloride were within20.00～400.00μg/ml(r=1.0000)and3.00～60.00μg/ml(r=0.9999)respectively.And the accuracy of these two components were within100.67%～101.40%and99.66%～101.64%respectively.The intra-day and inter-day RSD of these components were all less than5%.Conclusion:This method was simple,rapid and accurate,and suitable for quality control of large amounts of samples.

关 键 词：HPLC法 布洛芬 盐酸伪麻黄碱 

分 类 号：R917[医药卫生—药物分析学]