基质固相分散-高效液相色谱法测定蜂巢中7种黄酮类化合物  

Determination of 7 Flavonoids in Honeycomb by HPLC Combined with Matrix Solid Phase Dispersion

作　　者：李超[1] 王志兵[1] 杜婧[1] 邱芳萍[1] 

机构地区：[1]长春工业大学化学与生命科学学院,长春130012

出　　处：《理化检验（化学分册）》2015年第4期516-519,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：吉林省省校合作技术开发项目(721150)

摘　　要：采用基质固相分散-高效液相色谱法(MSPD-HPLC)同时测定蜂巢中芦丁、桑色素、木犀草素、槲皮素、芹菜素、白杨素和山奈素的含量。以硅胶为基质固相分散剂与样品混合均匀后装柱,以甲醇为洗脱剂进行洗脱。洗脱液在Pinnacle II C18分离柱上分离,以乙腈-0.2%(φ)磷酸溶液为流动相进行梯度洗脱,检测波长为260nm。7种黄酮类化合物的质量浓度在一定范围内与峰面积呈线性关系,检出限(3S/N)在0.07~0.32mg·L-1之间。方法用于测定蜂巢中7种黄酮类化合物,加标回收率在84.9%~95.9%之间,测定值的相对标准偏差(n=6)在2.3%~6.3%之间。A method of HPLC combined with matrix solid phase dispersion was used to measure Rutin、Morin hydrate、Luteolin、Quercetin、Apigenin、Chrysin,and Kaempferide in Honeycomb.Silica gel was used as matrix solid phase dispersing agent and mixed well with the sample to be used as column packing material.Then the seven flavonoids were eluted with methanol.The eluent was separated on a Pinnacle II C18 column using a mixture of acetonitrile-0.2%(φ)phosphate solution as mobile phase for gradient elution.UV-detection at 260 nm was adopted.The linear relationship between the peak area and the mass concentration of the seven flavonoids was in a certain range,with detection limits(3S/N)in the range of 0.07-0.32mg·L-1.The method was used to measure the seven flavonoids in honeycomb.The recovery rates measured by standard addition method were in the range of84.9%-95.9% with RSD(n=6)in the range of 2.3%-6.3%.

关 键 词：高效液相色谱法 基质固相分散 蜂巢 黄酮类化合物 

分 类 号：S89[农业科学—特种经济动物饲养] O657.72[农业科学—畜牧兽医]