青岛文昌鱼profilin样蛋白基因的克隆与同源性分析  

Cloning And Homology Analysis Of Amphioxus Profilin-Like Protein Gene In Qingdao Amphioxus

作　　者：张晓辉[1] 陈忠科[1] 林浴霜[1] 李琳[2] 杨焕明[2] 石德利[1] 张红卫[1] 

机构地区：[1]山东大学生命科学学院发育研究所,济南250100 [2]中国科学院北京华大基因研究中心,北京101300

出　　处：《山东大学学报（理学版）》2002年第6期561-565,共5页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金 ( 30 0 70 0 94);山东省自然科学基金 (L2 0 0 0D0 3) .

摘　　要：对青岛文昌鱼神经胚cDNA进行测序 ,获得了 6个profilin样蛋白基因的EST ,经拼接得到编码文昌鱼profilin样蛋白基因的cDNA序列并据此演绎了文昌鱼profilin样蛋白的氨基酸序列 .对演绎的文昌鱼profilin样蛋白的一级结构进行了分析 ,对其二级结构进行了预测 ,并将其与多种真核生物中的profilin进行同源性比较 ,为profilin分子进化的研究提供资料 .Profilins are low molecular mass,abundant cytosolic actin monomer binding proteins that bind actin,poly-L-proline (PLP),proline-rich proteins and membrane polyphopshoinositides.By sequencing the Qingdao amphioxus neurulae cDNA library,we obtained six expressed sequence tags(ESTs)of amphioxus profilin-like and got the full length cDNA sequence of.amphioxus profilin-like from these profilin-like ESTs.The deduced amino acid sequence was analyzed and compared with other animal and plant profilins' amino acid sequences.The secondary structure of amphioxus profilin-like was predicted.The results show that the profilin is conserved in evolution.

关 键 词：文昌鱼 PROFILIN 克隆 

分 类 号：Q754[生物学—分子生物学]