日本血吸虫可溶性虫卵抗原38kDa分子Dipstick快速诊断模式的初步建立  

Preliminarily establishing dipstick fast diagnostic system using soluble egg antigen (SEA) 38 kDa molecule of Schistosoma japonicum

作　　者：周晓红[1] 陈晓光[1] 戴琳[2] 胡旭初[1] 李华[1] 刘国章[1] 

机构地区：[1]第一军医大学寄生虫学教研室,广州510515 [2]南方医院感染内科,广州510515

出　　处：《热带医学杂志》2001年第1期25-27,共3页Journal of Tropical Medicine

基　　金：全军九五重点科研课题资助项目(No.96L001)

摘　　要：目的 建立简便的血吸虫病Ｄｉｐｓｔｉｃｋ快速诊断比方法 以纯化的日本血吸虫虫卵可溶性抗原（ＳＥＡ）３８ｋＤａ分子为诊断抗原，以胶体金标记的鼠抗人ＩｇＧ为二抗，建立 Ｄｉｐｓｔｉｃｋ快速诊断体系；检测日本血吸虫病人血清，急性期３７例、慢性期３０例，正常人５２例，肝吸虫病人血清３０例。结果 所测得的阳性率分别为９４．６％、８６．７％、１．９％、０％．伯论 以 ＥＡ ３８ｋＤａ纯化分子建立的血吸虫病Ｄｉｐｓｔｉｃｋ快速诊断法具有较好的敏感性和特异性，且简便快捷。Aim To establish convenient dipstick fast diagnostic system of schistosomiasis. Methods To establish dipstick fast diagnostic system, purified SEA 38 kDa molecule of .Schistosoma japonicum was used as diagnostic antigen and labeled mouse- anti- human IgG with colloidal gold was used as prode. These dipstick reagents were used to examine collected sera samples from 149 cases, including 37 acute Schistosomiasis patients, 30 chronic Schistosomiasis patients, 52 normal human controls anti 30 Clonorchiasis patients. Results The positive rates were 94. 6%, 86. 7% , 1. 9% and 0% respectively. Conclusion This dipstick fast diagnostic system using purified SEA 38kDa molecule antigen has high sensitivity and specificity. It is very convenient for field application.

关 键 词：日本血吸虫 可溶性虫卵抗原 38kDa抗原 免疫诊断 DIPSTICK 

分 类 号：R383.24[医药卫生—医学寄生虫学]