小鼠胸腺树突样细胞系与胸腺树突状细胞抗原提呈能力的比较研究  

Comparison studies on antigen presentation capability of a mouse thymic dendritic-like cell line and fresh thymic dendritic cells

作　　者：贺学英[1] 钱晓萍[1] 吴励[1] 陈慰峰[1] 

机构地区：[1]北京大学医学部免疫学系,卫生部医学免疫学重点实验室,100083

出　　处：《中华微生物学和免疫学杂志》2003年第2期127-131,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金重点项目 ( 39730 410 );香港青年学者合作研究基金项目 ( 3992 80 19)

摘　　要：目的　MTSC4(小鼠胸腺树突样细胞系 )能诱导胸腺细胞阴性选择 ,为阐明该功能是否与其抗原提呈能力有关 ,对MTSC4与新鲜胸腺树突状细胞 (T DC)的抗原提呈能力进行了比较研究。方法　应用MLR实验系统进行抗原提呈能力的比较分析 ;利用抗体阻断实验研究CD11c分子在抗原提呈中的作用。结果　T DC的抗原提呈能力分别是MTSC4、MTSC4 10 (MTSC4的 10号克隆 )、MTEC1(小鼠胸腺上皮细胞系 1)的 6 4、5 8、73倍 ,MTSC4、MTSC4 10、MTEC1之间APC能力无显著性差异。在联合细胞因子GM CSF ,IL 4,IFN γ和anti CD40McAb诱导下 ,MTSC4 10的表型变化显著 ,其MHCclassⅠ、MHCclassⅡ、B7.1表达阳性率分别由 10 %、阴性、10 %上调到 95 %、44 .7%、5 2 .7% ;MTSC4 10表型改变伴随抗原提呈能力增加 ,但仍只有T DC的 1/ 2 3。T DC与MTSC4 10的表型 ,抗原提呈能力的差异提示我们关注CD11c的作用 ,并首次证实anti CD11c单抗 (N418)能明显阻断新鲜分离T DC的抗原提呈作用 ,anti B7.2单抗对其抗体阻断有协同作用。结论　MTSC4的抗原提呈能力大大弱于T DC ;高表达于T DC的CD11c分子在抗原提呈中可能有重要作用。Objective To examine whether the MTSC4 (mouse thymic stromal cell 4, mouse thymic dendritic-like cell line) imposed negative selection on thymocytes is relevant to it′s antigen presentation capability, we compared the antigen presentation capability of MTSC4 with freshly isolated thymic dendritic cells. Methods Antigen presentation capability of different cells were measured by mix lymphocyte reaction (MLR) system, and antibody blocking assay was used to study the effect of CD11c molecule on antigen presentation. Results The antigen presentation capability of T-DC was 64-, 58- and 73-fold higher than those of MTSC4, MTSC4-10 (clone 10 of MTSC4) and MTEC-1 (mouse thymic epithelial cell-1), respectively. There was no significant difference in antigen presentation capability among MTSC4, MTS4-10 and MTEC-1. When three cell lines were stimulated by cytokines cocktail (GM-CSF, IL-4, IFN-γ) and anti-CD40 McAb, the expression levels of MHC class Ⅰ, class Ⅱ and B7.1 in MTSC4-10 were upregulated to 95%, 44.7%, 52.7%, respectively. The changes in phenotype were accompanied by the increase in antigen presentation capability of MTSC4-10, it was, however, still 23-fold lower than that of T-DC. Furthermore, anti-CD11c McAb (N418) significantly blocked the antigen presentation by T-DC. The inhibition was further deepened when anti-CD11c McAb was used in combination with anti-B7.2 McAb (GL1). Conclusions The antigen presentation capability of MTSC4 was much lower than that of fresh T-DC. The CD11c expressed on T-DC may function in antigen presentation. [

关 键 词：胸腺树突样细胞系 胸腺树突状细胞 抗原提呈 混合淋巴细胞培养 CD11C 免疫学 

分 类 号：R392[医药卫生—免疫学]