人TPO基因转染肝癌细胞后的表达及其受体水平的变化  

Expression of Recombinant hTPO Genes in HepG2 and Its Influences on the Expression of TPO Receptor

作　　者：柳光宇[1] 陈轶玉[2] 杨翔 霍天瑶[1] 黄成[1] 

机构地区：[1]南京大学生命科学学院,南京210093 [2]江苏职工医科大学,南京210000 [3]深圳市清华源兴生物医药科技股份有限公司,深圳518057

出　　处：《南京师大学报（自然科学版）》2003年第1期92-95,共4页Journal of Nanjing Normal University(Natural Science Edition)

摘　　要：从hTPO工程菌株中提取的pcDNA3/TPO质粒用脂质体包裹后转染人肝癌细胞株HepG2 ,并以未转染的HepG2和转染pcDNA3野生型质粒的HepG2作为对照 ,研究hTPO和其受体c mpl在三组细胞中的表达情况以及HepG2细胞的生长情况 ,发现 (1 )RT PCR法检测结果表明pcDNA3/TPO基因在转染HepG2细胞后 ,mRNA能有效表达 ,但对c mpl的表达基本无影响 ;(2 )细胞的生长计数和MTT法检测结果说明hTPO的有效表达对人肝癌细胞株HepG2的生长、繁殖有一定的抑制作用 。The expression of hTPO and c mpl (TPO receptor) in HepG2 was studied after hTPO genes were transferred to the cells.pcDNA3/TPO plasmids were extracted from recombinant bacteria DH5α and were transferred to HepG2 coated by liposome.The normal HepG2 and those transferred with pcDNA3 HepG2 were made as controls.By RT PCR,we found the gene of hTPO can be expressed effectively after transferred to HepG2,but it seemed to have no effects on the level of c mpl in HepG2.The results of MTT & density of HepG2 cells showed that the expression of hTPO can restrain the growth and proliferation of the HepG2,but we also found the transferring of liposome and pcDNA3 plasmids may stimulate the growth and proliferation of the HepG2.

关 键 词：TPO HEPG2细胞 血小板生成素受体 反转录PCR法 

分 类 号：Q754[生物学—分子生物学]