一株产黄酮银杏内生真菌的分离鉴定与培养介质的初步研究  

A Preliminary Study on the Isolation,Identification and Media of an Endophytic Fungus Producing Flavones

作　　者：王梅霞[1] 陈双林[1] 闫淑珍[1] 霍娟[1] 

机构地区：[1]南京师范大学生命科学学院,南京210097

出　　处：《南京师大学报（自然科学版）》2003年第1期106-110,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省教育厅自然科学基金项目 (02KJB1 80 0 0 2 );南京师范大学引进人才启动基金项目 (2 1 1 1 0 0B90 6)

摘　　要：从银杏 (GinkgobilobaL .)叶片中分离获得了一株内生真菌EG4 ,经形态分类学研究鉴定为刺盘孢Colletotrichumsp .用TLC分析培养液发现该菌可产生黄酮类化合物 .培养生物学特性的研究表明不同的碳、氮源对其生长和代谢有不同的影响 ,蔗糖和葡萄糖、NH+ 4,能够明显促进其生长 ;乳糖、NO- 2 能够明显促进其酮类物质的合成 .An endophytic fungus strain,i.e.EG4,was isolated from the leaves of Ginkgo biloba.It was identificated as Colletotrichum sp .based on morphological and taxonomic study.Furthermore,it was proved to produce flavone like compounds by TLC analysis.The culture characteristics of EG4 were also preliminarily studied.The results showed that tested carbon sources,nitrogen sources could affect EG4 growth and metabolism in different ways.Sucrose,glucose and NH + 4can clearly promote its growth; lactose and NO - 2can distinctly improve its ability to produce flavone like compounds.

关 键 词：银杏 内生真菌 黄酮类化合物 分离 鉴定 培养 

分 类 号：Q949.32[生物学—植物学] Q936