封闭式负压引流技术对大鼠创面愈合过程中VEGF和BCL-2的影响  

Effect of vacuum-assisted closure on expression of VEGF, Bcl-2 of rat wound

作　　者：汤苏阳[1] 陈绍宗[1] 胡昭华[1] 宋梅[1] 曹大勇[1] 吕晓星[1] 

机构地区：[1]第四军医大学唐都医院烧伤整形外科,陕西省西安市710038

出　　处：《中国美容医学》2003年第2期134-135,共2页Chinese Journal of Aesthetic Medicine

摘　　要：目的:研究封闭式负压引流技术(Vacuum-AssistedClosure,VAC.)对鼠失神经支配创面愈合中VEGF、Bcl-2表达的影响。方法:40只SD大鼠随机分成A、B两组(每组20只),于大鼠背部做一2cm×2cm的矩形全层皮肤缺损,其中A组创面予以封闭式负压引流组,B组未加封闭式负压引流;负压在20-80mmHg。在VAC后1,3,6,9,12天,A、B两组同时取材,采用免疫组织化学技术检测各组创面VEGF(Vascularendothelialgrowthfactor,VEGF)、Bcl-2的表达和标记指数。结果:A组较B组其VEGF、Bcl-2表达及标记指数明显增加(P<0.001)。结论:VAC能明显促进创面的修复细胞的增殖和血管生成。Objective: To investigate the effect of Vacuum-Assisted Closure on Expression of VEGF, Bcl-2 of rat wound healing. Methods:40 Sprague-Dawley rats were randomly and equally divide into A group, B group (20 in each group), that A group was employed the technique of Vacuum-Assisted Closure the B group as the controls ,in which the A,B group were cut the back ,the wound.scope was about 2cm×2cm.VAC was adopt at 80mmHg. At the1,3,6,9,12days after VAC, the expression of vascular endothelial growth factor and Bcl-2 were detected by immunohistochemistry method. Results: The expression and Labelling index of VEGF, Bcl-2 were much more in A group than that in B group at all check time points(P< 0.05). Conclusion:The technique of vacuum-assisted closure might obviously facilitate the proliferation of vascular endothelial cell and angiogenesis of rat wounds.

关 键 词：封闭式负压引流技术 失神经支配创面 血管生成 细胞增殖 VEGF BCL-2 动物实验 

分 类 号：R641[医药卫生—外科学]