有机锗(Ge-132)对培养黑素细胞的影响  

The Study of the effection of Ge-132 on Cultured Melanocytes

作　　者：牟宽厚[1] 冯捷[1] 

机构地区：[1]西安交通大学第二医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2003年第2期91-92,共2页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助 (30 2 0 0 2 4 7)

摘　　要：目的　探讨有机锗 (Ge 13 2 )对培养的黑素细胞的作用。方法　采用经典的黑素细胞培养方法并加以改良取得第二代完全纯化的黑素细胞 ,按浓度梯度方法向每组培养物中加入不同浓度的药物 ,检测细胞的增殖活性。结果　当培养基中Ge 13 2浓度大于 0 .0 8μg/m1时 ,细胞生长呈现以下变化 :细胞增殖活性下降 (P <0 .0 0 1) ;酪氨酸酶活性受到抑制 (P <0 .0 0 1) ;黑素细胞内黑素含量下降 (P <0 .0 0 1)。结论　Ge 13 2在较低浓度 ( >0 .0 8μg/ml)时对黑素细胞的生长有抑制作用。Objective To explore the functions of Ge-132 on cultured melanocytes.Methods Adopting the classic ways of culturing melanocytes and improving it,acquiring the second generation of pured melanocyte,adding drugs at different concentrations to every medium, checking the activity of the cells.Results By the concentrations more than 0.08 μg/ml (per media), the multiplication of the melanocytes becomes low (P<0.001),The activity of tyrosinase is inhibited(P<0.001).The melanin goes down (P<0.001).All had marked difference.Conclusion Ge-132 can inhibit proliferation of melanocytes at lower concentration.

关 键 词：有机锗 GE-132 培养 黑素细胞 影响 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]