NANOPARTICLE AS A NEW GENE TRANSFERRING VECTOR IN SPECIFIC EXPRESSION GENE  

作　　者：管珩 李拥军 郑曰宏 刘昌伟 杨菁 宋存先 王彭延 赵三妹 王宗立 佘铭鹏 

机构地区：[1]Department of General Vascular Surgery, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730 [2]Institute of Biomedical Engineering, CAMS & PUMC, Tianjin 300192 [3]Department of Pathology, Institute of Basic Medical Sciences,CAMS & PUMC, Beijing 100005

出　　处：《Chinese Medical Sciences Journal》2002年第4期220-224,共5页中国医学科学杂志（英文版）

基　　金：This program was supported by the National Natural Sciences Foundation of China (No. 39870196).

摘　　要：Objective. To evaluate the possibility and efficiency of nanoparticle as a new vector in specific gene transference.Methods. Nanoparticle-DNA complex was prepared with Poly- dl-lactic-co-glycolic acid (PLGA) bearing anti-sense monocyte chemotactic protein-1 (A-MCP-l), a specific expression gene, and the package efficiency, release progress in vitro, and the size of the complex were determined. The possibility of the new vector was evaluated with genomic DNA PCR by transferring gene into cultured smooth muscle cells (SMC), cationic lipids as a control. For study in vivo, jugular vein-to-artery bypass grafting procedures were performed on 20 New Zealand white rabbits, of which 6 grafts were transferred with nanoparticle-A-MCP-1 (200 μg), 6 with A - MCP-1 (200 μ g) by cationic liposome, 4 with LNCX plasmid, and 4 as control. Fourteen days after the grafts were harvested, the expression of A-MCP-l and its effect on MCP-1 in vein grafts were detected by dot blot, and the morphologic evaluation of grafts was performed.Results. The package efficiency of the nanoparticle-DNA complex was 0. 9% , release progress in vitro lasted 2 weeks, and the size ranged from 150 to 300nm. SMC genomic DNA PCR showed that A-MCP-l gene could be successfully transfected into cells by nanoparticle. The study in vivo indicated that A-MCP-l mRNA was expressed in both local gene delivery groups, nanoparticle and liposome, meanwhile, MCP-1 expression in vein grafts was significantly inhibited and neointimal hyperplasia was notably reduced.Conclusion. Nanoparticle can act as a vector to transfect specific gene.

关 键 词：gene therapy NANOPARTICLE monocyte chemotactic protein-1 

分 类 号：R346[医药卫生—基础医学]