广西巴马长寿乡壮族长寿老人永生细胞库的建立  

ESTABLISHING THE CELL-STORAGE OF IMMORTAL CELL LINES FROM THE LONGVOUS RESIDENTS OF ZHUANG NATIONALITY IN LONGEVITY VILLAGES IN BAMA, GUANGXI

作　　者：郑陈光[1] 龙桂芳[2] 杨泽[3] 吕泽平[1] 梁积英[1] 潘尚领[2] 吴建洲[1] 胡才友[1] 韦勇[1] 李旭[1] 陈进超 许代娣[2] 史晓红[3] 孙玉茹[3] 庞国防[1] 阳初玉[1] 

机构地区：[1]广西壮族自治区江滨医院,南宁530021 [2]广西医科大学 [3]北京医院卫生部老年医学研究所遗传室 [4]广西巴马县长寿研究所

出　　处：《广西医科大学学报》2003年第1期5-6,共2页Journal of Guangxi Medical University

基　　金：广西自然科学基金项目 (桂科自 9912 0 32 )资助

摘　　要：目的 :建立广西巴马长寿乡壮族长寿老人永生细胞库。方法 :使用 EB病毒转化外周血 B淋巴细胞方法。结果 :建立了长寿者外周血细胞转化永生细胞株的方法 ,转化成功率 77.4 %。建立了 65株长寿老人、直系亲属永生细胞株及长寿老人资料库和完整的细胞株档案。结论 :广西巴马长寿乡壮族长寿老人永生细胞库的建立为永久保存细胞系及后续研究提供了必要的基础。Objective:To establish a cell storage of immortal longevity cell lines from centenarians,nonagenarians living in Zhuang nationality in longevity villages in Bama, Guangxi.Method:The research was conducted by way of peripheral B lymphocytes transformed by Epstain Barr Virus. Result:We used the method to obtain immortal longevity cell lines from peripheral B lymphocytes transformed by Epstain Barr Virus at the success rate of 77 4 %,65 lines of immortal longevity cell lines from the centenarians and nonagenarians were obtained successfully. We also got relative information data about these peoples and entire data for the cell storage.Conclusion:Establishing the cell storage of immortal longevity cell lines from centenarians,nonagenarians living in Zhuang nationality in longevity villages in Bama,Guangxi.Our work will provide the necessary basis for the permanent preservation of these cell lines as well as successive studies.

关 键 词：广西 巴马长寿乡壮族 长寿老人 永生细胞库 建立 

分 类 号：R339.36[医药卫生—人体生理学]