脑缺氧缺血后新生大鼠海马CA_1区NMDAR的表达  

Study of NMDAR expression in hippocampal CA_1 region in neonatal rats with hypoxic-ischemia

作　　者：曹艳华[1] 王玲[1] 曹玉红[2] 王宝西[1] 鱼绥和[1] 

机构地区：[1]第四军医大学唐都医院儿科,陕西西安710038 [2]第四军医大学西京医院儿科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2003年第2期153-155,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的 :研究新生大鼠缺氧缺血性脑损伤 (HIBD)后 ,海马CA1区N甲基D天门冬氨酸受体 (NMDAR)及NMDARmRNA表达的变化。方法 :建立HIBD模型 ,用免疫组化及原位杂交方法 ,检测正常对照 (NORM)组和缺氧缺血 (HI)后不同时间点 ,NMDA受体I型亚单位 (NR1)表达的阳性细胞及NR1mRNA表达的阳性细胞。结果 :HI后 2h时NR1、NR1mR NA的表达稍下降 ,2 4h开始上升 ,72h达高峰 ,与正常对照组相比较有显著意义 (P <0 .0 5 )。结论 :正常新生大鼠海马CA1区有NR1及NR1mRNA的表达 。AIM: To study the expressions of NMDAR in hippocampal CA 1 region after hypoxic ischemic brain damage(HIBD) in neonatal rats. METHODS: An neonatal rat model with hypoxic ischemic brain damage was set up. The expression of NMDAR was detected in normal control and hypoxic ischemic (HI) brain damage group by immunohistochemical staining and in situs hybridization. RESULTS: The number of NR1 + and NR1 mRNA + cells decreased slightly in hippocampal CA 1 region at 2 h after HI. The numbers increased from 24 h to 48 h after HI, and reached maximum at 72 h after HI . CONCLUSION: NR1 expressed in hippocampal CA 1 region of normal neonatal rats, and the expression is up regulated after HI brain injury.

关 键 词：脑缺氧缺血 新生大鼠 N甲基D天门冬氨酸受体 兴奋性氨基酸 基因表达 

分 类 号：R722.12[医药卫生—儿科]