新生大鼠海马神经元原代培养方法的研究  

The study of primary culture methods for hippocampal neurons of newborn rats

作　　者：王英[1] 车宇[1] 苗建亭[1] 李柱一[1] 王者晋[1] 

机构地区：[1]第四军医大学唐都医院全军神经内科中心,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2003年第2期197-199,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：军队"十五"医药卫生科研基金资助项目 (No .0 1Q1 1 2 );第四军医大学创新工程基金资助项目 (No .CX0 1A0 1 8)

摘　　要：目的 :研究和比较 3种不同培养神经元的方法 ,提供一种纯化效率和存活率均较高的培养技术。方法 :取新生SD大鼠的海马区 ,应用一般培养法、加阿糖胞苷法、加阿糖胞苷和神经生长因子法培养神经元 ,观察神经元的形态。用倒置显微镜观察和MTT比色分析检测神经元的存活率。用ABC免疫组化染色法 ,比较 3种不同培养方法在不同培养时间所获得神经元的纯度。结果 :加阿糖胞苷和神经生长因子培养组神经元生长良好 ,纯度和存活率均较高。结论 :加阿糖胞苷和神经生长因子培养神经元的方法具有简便可行 ,易于生长的优点 。AIM: To compare three different methods for neuron culture, so as to provide a culture technique with higher neuron purity and survival rate. METHODS: Neurons in hippocampal region of newborn SD rats were cultured by common culture method, cytosine arabinoside (Ara c) supplementing method, Ara c and nerve growth factor (NGF) supplementing method. Then morphology of neurons was observed under microscope. The survival rates of the neurons at different culture times were compared by inverted microscope observation and MTT colorimetry. The purity of neurons cultured by 3 methods was detected by immunocytochemical staining. RESULTS: Neurons cultured by Ara c and NGF supplementing method grew well. The purity and survival rate of neurons cultured by Ara c and NGF supplementing method was highest. CONCLUSION: Ara c and NGF supplementing method is a good method for neuron culture.

关 键 词：新生大鼠 神经元 细胞培养 一般培养法 加阿糖胞苷法 神经生长因子 

分 类 号：R392－33[医药卫生—免疫学]