检测抗人疱疹病毒6型IgG的间接免疫荧光试验的建立及其应用  

Development and application of an indirect immunofluorescence assay for the detection of serum immunoglobulin G to human herpesvirus 6

作　　者：刘军连[1] 徐志凯[2] 王红英[3] 王德堂[1] 张芳琳[2] 杨乔欣[2] 姚堃[4] 

机构地区：[1]中国人民解放军第306医院皮肤科,北京100101 [2]第四军医大学微生物学教研室 [3]第四军医大学西京医院妇产科 [4]南京大学医学院微生物学教研室

出　　处：《细胞与分子免疫学杂志》2003年第2期181-182,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助 (No .392 70 0 34);江苏省自然科学基金资助 (No .BJ930 96)

摘　　要：目的 :建立检测血清中人疱疹病毒 6型 (HHV 6 )IgG的间接免疫荧光试验 (IFA)。方法 :用HHV 6国内分离株感染人脐带血单个核细胞制备抗原片 ,建立检测HHV 6IgG的IFA方法 ,并用于育龄期妇女血清流行病学调查。结果 :建立的IFA具有特异性。对 116份育龄期妇女血清标本检测表明 ,HHV 6IgG的阳性率为 72 .4 % ,几何平均滴度 (GMT)为 1∶6 1;在孕妇和正常未孕妇女之间 ,以及不同孕期的孕妇之间 ,HHV 6IgG的阳性率和GMT均无差异 (P >0 .0 5 )。结论 :建立了具有特异性的IFA法 ,可用于对育龄妇女HHVAIM: To establish an indirect immunofluorescence assay(IFA) for detection of serum IgG antibody against human herpesvirus 6(HHV 6). METHODS: Cord blood mononuclear cells infected by HHV 6 strain CN 5 were used to prepare cell antigen smear, so as to establish an IFA and make an epidemical investigation on serum specimens of child bearing age, women. RESULTS: The specificity of the IFA for HHV 6 was confirmed by absorption assay(test). The IFA detection showed that in serum specimens from 116 cases of child bearing age, women, the positive rate of anti HHV 6 IgG was 72.4% and geometric mean titer(GMT) was 1∶61. The positive rate and GMT of serum anti HHV 6 IgG had no differences between pregnant women and unpregnant women, neither among pregnant women at different pregnant stages. CONCLUSION: A specific IFA has been developed successfully for epidemical investigation of HHV 6 infection rate in child bearing women.

关 键 词：人疱疹病毒6型 间接免疫荧光试验 IGG抗体 育龄期妇女 血清流行病学调查 

分 类 号：R752.1[医药卫生—皮肤病学与性病学] R373.11[医药卫生—临床医学]