E-test试条对异质性耐万古霉素葡萄球菌的检测  

Significance of E-test strip in identification of heterogeneously vancomycin-resistant Staphylococci spp.

作　　者：吴本权[1] 唐英春[1] 张扣兴[1] 张天托[1] 饶宪[1] 毕筱刚[1] 周凤丽[1] 

机构地区：[1]中山大学附属第三医院,广州510630

出　　处：《中国抗生素杂志》2003年第4期249-251,共3页Chinese Journal of Antibiotics

基　　金："2 0 0 1年罗氏感染研究青年医师基金"资助

摘　　要：目的　探讨E test对异质性耐万古霉素葡萄球菌的检测价值。方法　从连续接触万古霉素的脑心浸液琼脂 (BHIA)平板上挑取万古霉素异质性耐药葡萄球菌菌株 ,应用万古霉素E test试条、药敏纸片和琼脂平板对倍稀释法测定药敏结果。结果　E test检测的对万古霉素异质性耐药葡萄球菌MRSH10 9,MRSH2 ,MRSH13 ,MRSH 3 1,MRSH 60 ,MRSH 10 8,MRSA73 ,MRSA97,MRSA10 2的MIC依次为 :16、2 4、48、48、16、3 2、2 4、2 4mg/L ,其中耐药 7株占 77 8% ( 7/9) ,中介 2株占 2 2 2 % ( 2 /9) ;琼脂对倍稀释法的MIC为 :16、3 2、64、3 2、16、3 2、3 2、16、3 2mg/L ,耐药 6株占 66 7% ( 6/9) ,中介 3株占 3 3 3 % ( 3 /9) ;药敏纸片直径依次为 :14、12、10、11、10、15、13、10 ,13mm ,敏感率为 11 1% ( 1/9)。结论　E test可以便捷直观地鉴定对万古霉素异质性耐药葡萄球菌的药敏结果 ,与琼脂平板对倍稀释法接近 ,优于纸片法。Objective To evaluate the value of E-test strip in the identification of heterogeneously vancomycin-resistant Staphylococci spp. Methods the heterogeneously vancomycin-resistant strains were selected from the Brain Heart Infusion agar plate with continuous exposure to vancomycin. E-test strip, disk diffusion and agar dilution method were used to detect the susceptibility of these bacteria. Results E-test showed that the MIC of heterogeneously vancomycin-resistant Staphylococci MRSH109, MRSH2, MRSH13, MRSH31, MRSH60, MRSH108, MRSA73, MRSH97, MRSA102 were 16, 24, 48, 48, 16, 32, 32, 24, 24mg/L, respectively , in which 77.8% (7/9) of strains were resistant and 22.2% (2/9) intermediate. The agar dilution method showed that the MIC of these bacteria were 16, 32, 64, 32, 16, 16, 32, 32, 32mg/L, respectively of these bacteria, 66.7% (6/9) of strains were resistant and 33.3% (3/9) intermediate. The zone diameter were 14, 12, 10, 11, 10, 15, 13, 10, 13mm, respectively, in which 11.1% (1/9) of strains were sensitive. Conclusion E-test can conveniently identify the heterogeneously vancomycin resistant Staphylococci similar to the agar dilution method and superior to the paper disks.

关 键 词：万古霉素 抗药性 葡萄球菌 检测 E-test试条 

分 类 号：R978.1[医药卫生—药品] R378[医药卫生—药学]