小剂量强地松、红霉素和富露施联用对大鼠肺纤维化及其转化生长因子β_1干预的实验观察  

AN EXPERIMENTAL OBSERVATION OF PULMONARY FIBROSIS IN RATS AND ITS TGFβ_1 IN SMALL DOSE OF PREDNISONE AND ERYTHROMYLIN AND N-ACETYLCYSTEINE

作　　者：雷丰丰[1] 李惠菊[1] 张启昆[1] 万毅新[1] 郑宁[1] 

机构地区：[1]兰州医学院第二附属医院呼吸科,甘肃省兰州市730046

出　　处：《中国煤炭工业医学杂志》2003年第5期478-480,共3页Chinese Journal of Coal Industry Medicine

摘　　要：目的　提供更合理的肺纤维化治疗方案。方法　 1 0 0只wistar大鼠 ,随机抽出 2 0只作为正常组 ,气管内注入生理盐水 (作为阴性对照 ) ,剩余 80只经气管内注入博莱霉素造模成功后随机分为模型组 2 0只 (作为阳性对照 ) ,大剂量强地松组 2 0只 ,红霉素和富露施组 2 0只 ,小剂量强地松、红霉素和富露施组 2 0只 ,分别给予相应药物治疗。以上各组动物在第七 ,十四 ,三十 ,六十天每组随机抽出5只处死进行病理切片观察 ,电子计算机图像分析仪进行组织形态学、胶原和转化生长因子 (TGF) β1 定量分析。结果　小剂量强地松、红霉素和富露施组对模型动物干预最强。结论　小剂量强地松、红霉素和富露施联合应用治疗大鼠肺纤维化优于经典方法。Objective To provide a better way to treat the bleomycin-induced pulmonary fibrosis. Methods Choosing randomly 20 of 100 wistar rats as normal control group, the rests with beomycin A5 induced fibrosis were divided randomly into 4 groups.5 rats of every group were put to death and made sections of lungs with HE or Masson staining and immunohistochemistry after 7,14,30,60 days.Histopathological examination and image processing computer were assisted to evaluate the results of treatments. Results The group treated by small dose of prednisone and erythromycin and N-acetylcysteine group has the most intensive effects in the 3 treatment groups. Conclusion The small dose of prednisone and erythromycin and N-acetylcysteine in the Bleomycin-induced Pulmonary Fibrosis of rats has better effect than traditional treatment.

关 键 词：小剂量 强地松 红霉素 富露施 联用 大鼠 肺纤维化 转化生长因子β1 发病机制 

分 类 号：R563.13[医药卫生—呼吸系统]