补肾益气活血方对宫内发育迟缓胎鼠肝脏IGF-Ⅰ表达的影响  

Effect of Reinforcing Kidney, Replenishing qi and Promoting Blood Circulation Recipe on the Expression of IGF-Ⅰ in Liver of the Fetal Rat with IUGR

作　　者：王琪[1] 黄光英[1] 袁永辉[2] 李臣鸿[3] 熊丽萍[2] 

机构地区：[1]华中科技大学同济医学院中西医结合研究所,武汉430030 [2]华中科技大学同济医学院基础医学院病理学系,武汉430030 [3]华中科技大学生命科学与技术学院,武汉430074

出　　处：《华中科技大学学报（医学版）》2003年第2期212-215,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No. 39870 92 3)

摘　　要：目的　观察补肾益气活血方对宫内发育迟缓 (intrauterine growth retardation,IU GR)胎鼠肝脏胰岛素样生长因子 - 1(insulin like growth factor- ,IGF- )基因在转录和翻译水平上的影响 ,探讨其防治 IU GR的作用机制。方法　采用被动吸烟法复制胎鼠 IU GR模型 ,分为正常对照组、 IU GR组、 IUGR精氨酸治疗组 (精氨酸组 )、 IU GR补肾益气活血方治疗组 (中药组 )。采用 Northern blot和免疫组织化学的方法分别检测胎鼠肝脏 IGF- m RNA和蛋白的表达。结果　 IUGR组 IGF- m RNA及蛋白表达水平与对照组比较明显下调 (P<0 .0 1) ,而精氨酸组和中药组两者表达水平较 IUGR组有所增加 ,其中 ,中药组 IGF- 的表达强度高于精氨酸组 (P<0 .0 5 ) ,接近正常组 (P>0 .0 5 )。结论补肾益气活血方可能通过直接 (或间接 )促进 IGF- 的合成和分泌 ,或者防止某些致病因素对 IGF- 基因表达细胞信息传导途径的阻断而起到防治Objective To investigate the effect of reinforcing kidney, replenishing qi and promoting blood circulation recipe on the transcription and translation of IGF Ⅰ gene in the liver of the fetal rats with intrauterine growth retardation (IUGR). Methods The smoke inhalation IUGR animal model was established, and the pregnant Sprague Dawley rats were randomly assigned to 4 groups: control group, IUGR group, group of IUGR treated with reinforcing kidney, replenishing qi and promoting blood circulation recipe (Chinese medicine group), group of IUGR treated with L arginine ( L arginine group). The expression of IGF Ⅰ mRNA and IGF Ⅰ protein in rat liver was detected by Northern blot and immunohistochemistry respectively. Results By Northern analysis, the level of IGF Ⅰ mRNA was decreased significantly in IUGR group as compared with other three groups. Immunohistochemistry analysis revealed that the expression of IGF Ⅰ protein in the IUGR group was decreased as compared with other groups ( P <0 05 or P <0 01), and the immunoactivity of IGF Ⅰ was increased more significantly in Chinese medicine group than in L Arg group ( P <0 05), but there was no significant difference between Chinese medicine group and control group ( P >0 05). Conclusion The effect of Chinese medicine on preventing and treating IUGR may be attributed to regulating the synthesis and excretion of IGF Ⅰ directly or indirectly, and preventing the action of some etiologicat factors on interrupting the pathway of cell signal transduction correlated with the expression of IGF Ⅰ gene.

关 键 词：宫内发育迟缓 补肾益气活血中药 肝脏胰岛素样生长因子-1 作用机制 信息传导途径 

分 类 号：R271.944.31[医药卫生—中西医结合]