用原位核酸杂交技术探讨肠道病毒与急型克山病的关系  

Relationship between enteroviruse s and acute Keshan disease in Jilin province by using nucleic acid hybridization in situ

作　　者：李相军[1] 任立群[1] 李广生[1] 

机构地区：[1]吉林大学生物工程研究所,长春130021

出　　处：《中国地方病防治》2003年第2期72-73,共2页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金资助项目(39870668)

摘　　要：目的探讨肠道病毒(柯萨奇病毒)与吉林省急型克山病的关系。方法用柯萨奇病毒B3、B4、B5、A9共同序列作寡核苷酸探针对吉林省急型克山病尸检心肌组织12例及病区与非病区尸检正常心肌组织各7例进行原位核酸杂交。结果吉林省急型克山病12例中有11例出现阳性杂交信号,即有肠道病毒RNA存在,阳性率为91.66%,对照组均为阴性。结论吉林省急型克山病病人心肌组织中有肠道病毒感染,提示急型克山病的发病可能与肠道病毒感染有关。Objective To study the relationship between en teroviruses and acute Keshan disease in Jilin province.Method We used hybridization in situ to exam ine RNA of enteroviruses in paraffin-embedded myocardial specimens from 12patients with acute Keshan di sease and 14patients who had no heart disease,7from endemic area and the o thers from non -endemic area.Result Of the 12patients with acute Keshan d isease,11patients(91.66%)showed positive findings.Their myocardial tissues were infected by enteroviruses previously.The cont rol group had no positive fingdings.Conclusion The re-sults suggested that enteroviruses infection might be one of the major ca uses of acute Keshan disease in Jilin province.[

关 键 词：肠道病毒 柯萨奇病毒 急型克山病 关系 原位核酸杂交技术 

分 类 号：R373.2[医药卫生—病原生物学] R542.3[医药卫生—基础医学]