金属离子对淀粉酶催化抑制作用的研究  

STUDY ON INHIBITION OF METAL IONS TO AMYLASE CATALYZED REACTION

作　　者：于秀芳[1] 

机构地区：[1]曲阜师范大学化学系,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2003年第2期77-79,共3页Journal of Qufu Normal University(Natural Science)

摘　　要：利用微量量热法测定了无竞争性抑制和有竞争性抑制时淀粉在淀粉酶催化作用下的热功率—时间曲线 ,借助热动力学理论和对比进度法对曲线进行处理 ,得到了无竞争性抑制时酶催化反应的米凯利斯常数 (Km)和最大速率 (Vmax) ,以及有竞争性抑制 (Ni2 +)时酶催化反应的表观米凯利斯常数 (K1m)和最大速率(Vmax) .建立了Km 与金属离子浓度间的关系 ,即K1m=2 .96 5 6× 10 -3 +1.394× 10 -4 C .Power-time curves of amylase catalyzed reaction with or without competitive inhibition were obtained with 2277 thermal activity monitor (Sweden). The K m and V max of enzyme catalyzed reactions without competitive inhibitors, apparent and V max with competitive inhibitors (Ni 2+) were gained by investigating curves with thermokinetics theory and reduced extent method. The relationship between K-m and concentration of metal ions(C) was established and so did the rule of inhibition of metal ions to amylase catalyzed reaction.

关 键 词：淀粉酶 催化抑制作用 金属离子 酶催化反应动力学 微量量热法 热动力学 对比进度法 

分 类 号：Q556.203[生物学—生物化学]