转基因处理过程中甜瓜黄化不定芽转绿因素初探  

Initial Studies of Factors in Turning Yellowed Adventitious Buds into Green During Treatment of Transgenic Muskmelon

作　　者：计巧灵[1] 李冠[1] 王卫国[1] 骆建敏[2] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]新疆大学理化测试中心,新疆乌鲁木齐830046

出　　处：《新疆大学学报（自然科学版）》2003年第2期184-187,共4页Journal of Xinjiang University(Natural Science Edition)

摘　　要：以甜瓜品种红蜜脆、皇后的无菌子叶块为外植体,用农杆菌介导法进行遗传转化处理.针对遗传转化处理后,在外植体上长出的甜瓜不定芽发生黄化的现象,从品种差异、遗传转化条件、矿物营养含量、光照强度、温度等多方面作了初步探讨.结果表明:与未遗传转化处理的子叶块上长出的不定芽相比,转化处理后的子叶块上长出的不定芽黄化现象普遍.将后者分别转接入多添加了Fe或Mg或B或K盐的改良芽生长培养基上培养,一段时间后,形态观察表明,在多添加了Fe盐的培养基上不定芽转绿明显,其次,在多添加了Mg盐的培养基上不定芽也有转绿.显微、超微结构上显示,在多添加了Fe盐的培养基上不定芽叶栅栏细胞内含较多的叶绿体,其叶绿体内有丰富的光合片层结构.光照强度对不定芽转绿作用不明显,温度保持恒定(270C+20C)或波动(150C～350C)对甜瓜不定芽转绿影响不大,两个品种在这方面的差异很小.This paper reported that the explants of bacteria-free cotyledons of Xinjiang muskmelon was trans-geneted treatment through Agrobacterium tumefaciens. Be directed against the yellowing leaves of adventitious buds after trangenic treatment,we engaged explorations from strain variance and conditions of genetic transformation and contents of mineral nutritious and intensity of illumination as well as temperation. The results indicated that the adventitious buds,from the explants of transgenic treatment , are yellowing universally by contrast. We took them respectively on the improve mediums of bud-growth which added Fe or Mg or B or K salt. After a period of time , the results indicated from morphological observation that the adventitious buds grew on the medium added Fe salt became green and green evidently, and so did the bud on the medium added Mg salt. The results indicated from micro and super-micro structures that the palisade cells of adventitious buds from medium added Fe salt contain many more chloroplasts, and also there are a lot of photosynthetic lamellaes are rich in the chloroplast.

关 键 词：甜瓜 不定芽 黄化 转绿因素 遗传转化处理 转基因作物 矿物营养 抗病育种 

分 类 号：S652.034[农业科学—果树学] Q943.2[农业科学—园艺学]