RT-PCR检测不同时间大鼠皮肤切创TGF-β_1mRNA表达  

An assay of RT-PCR on the time-related expressions of TGF-β_1 mRNA in rat skin wounds

作　　者：王慧君[1] 丁云川[1] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515

出　　处：《中国法医学杂志》2003年第2期67-69,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金(编号39870764);广东省科委攻关项目(2KM054045;2000)

摘　　要：目的 探讨TGF-β_1mRNA在不同时间的表达变化及其与伤后经过时间的关系。方法 应用逆转录-聚合酶链反应(RT-PCR)分别对大鼠生前0.5h、1h、3h、6h、12h、48h、72h、96h、168h和死后0.5h、1h、3h的皮肤切创组织中的TGF-β_1mRNA表达变化进行检测,采用ID软件对凝胶扫描的数据进行密度分析。结果 TGF-β_1mRNA在生前皮肤切创后0.5h上升,3h开始急剧升高,48h含量达到峰值;死后皮肤切创未见TGF-β_1mRNA的表达。结论 大鼠皮肤切创TGF-β_1mRNA的表达具有时间相关性,RT-PCR是检测细胞因子在基因水平表达的灵敏方法。Objective To explore the time-related expressions of TGF-β1mRNA during the healing process of rat skin wounds. Method Using the method of the reverse transcriptase polymerase chain reac-tion (RT-PCR) on intra-vital rat skin incised wounds (0.5h, 1h, 3h, 6h, 48h, 72h, 96h, 168h after incision) and postmortem rat skin incised wounds (0.5h, 1h, 3h after incision) to detect the dynamics of expression Ievel of TGF-β1mRNA. The strength of the expression of TGF-β1mRNA in scanned image was using ID-Advanced software. Results The results of RT-PCR showed that TGF-β1mRNA Ievel increased at 0.5h after incision and elevated significantly after 3h. The peak of TGF-β1mRNA occurred at 48h. There was no obvious expression of TGF-β1mRNA in postmortem incised wounds. Conclusion The characteristics of the TGF-β1mRNA expression were potentially indicative for the wound aging. RT-PCR was a sensitive method for detection of the expression of cytokines in genic level.

关 键 词：法医学 损伤时间 转移生长因子Β1 基因表达 逆转录-聚合酶链反应 

分 类 号：D919[医药卫生—法医学]