转化生长因子βⅡ受体在大鼠胰腺发育后期的表达  

Expression of TGF-βII Receptor during the Later-stage Development of Rat Pancreas

作　　者：熊俊[1] 程志祥[1] 倪雪峰[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学与分子生物学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2003年第3期240-242,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助项目(BK2001119)

摘　　要：目的:探讨转化生长因子(TGF)-βⅡ受体在SD大鼠胰腺发育后期的表达及意义。方法:使用RT-PCR方法分别检测交配后18.5天、新生及成年SD大鼠胰腺TGF-βⅡ受体mRNA表达;使用Western blot方法分别对上述不同时期SD大鼠胰腺TGF-βⅡ受体表达进行蛋白质水平的检测。结果:新生鼠胰腺TGF-βⅡ受体表达在核酸与蛋白质水平上均明显高于18.5天胎鼠与成年鼠胰腺,差异有显著性(P<0.05)。结论:TGF-βⅡ受体表达在新生鼠阶段有明显上调。Objective: To analyse the role of TGF-βⅡ receptor during the later-stage development of pancreas in SD rat. Methods: The mRNA of TGF-βⅡ receptor was amplified by reverse transcription PCR and the expression of TGF-βⅡ receptor protein was investigated by Western blot. Results: The mRNA and protein expression of TGF-βⅡ receptor of newborn rat pancreas is significantly higher than those of embryo rat of 18. 5 days and adult rat pancreas respectively (P < 0. 05). Conclusion: There is a significant upregula-tion of TGF-βⅡ receptor during the later-stage development of rat pancreas.

关 键 词：转化生长因子BⅡ受体 胰腺 逆转录-聚合酶链反应 免疫印迹法 

分 类 号：R392.11[医药卫生—免疫学]