微柱凝胶间接抗球蛋白法筛选和鉴定不规则抗体  

Micro-Column Gel Indirect Anti-globulin Technique for Screening and Identification of Irregular Antibody

作　　者：马曙轩[1] 刘景汉[1] 李锡金[1] 骆群[1] 陈民才[1] 王海军[1] 

机构地区：[1]解放军总医院输血科,全军临床输血中心,北京100853

出　　处：《中国实验血液学杂志》2003年第2期194-196,共3页Journal of Experimental Hematology

摘　　要：为了建立微柱凝胶间接抗球蛋白法筛选与鉴定不规则抗体的实验方法 ,探索采用血浆代替血清进行抗体筛选的可行性 ,采用多人份标准红细胞混合检测不规则抗体法 ,并与传统抗球蛋白法加木瓜蛋白酶两步法进行了对比 ;采用微柱凝胶间接抗球蛋白法对我院受血者抗凝标本进行抗体筛选。对筛检阳性的受血者首先采用微柱凝胶间接抗球蛋白法进行抗体鉴定 ,然后采用传统抗球蛋白法加木瓜蛋白酶两步法再次进行抗体鉴定。抗体鉴定所用的标准细胞均为 11个标准细胞的单人份混悬液。研究结果显示 ,采用微柱凝胶间接抗球蛋白法混合标准细胞筛检 5 0 0 0份受血者标本 ,共检出不规则抗体 2 0例 ,阳性率为 0 .4 % ,其中抗 D 2例 ,抗 E 8例 ,抗 C 1例 ,抗 c 2例 ,抗 Mia2例 ,抗 Jka2例 ,抗 Lea1例及抗 Fya2例。采用传统抗球蛋白法检出了其中的 19例 ,而木瓜蛋白酶法检出其中的 13例。抗 Lea 抗体经加入Le(a b )型人的补体后也检出。结论 :微柱凝胶间接抗球蛋白法混合细胞筛检受血者不规则抗体可以取代传统抗人球加酶法在临床大批量推广应用 。The purpose of this study was to design an antibody screening method based on the micro column gel indirect anti globulin technique (MGIAT), using pooled cells and plasma, by comparison with the conventional indirect anti globulin technique(CIAT) combined with a two stage papain technique, and to explore the feasibility of the use of plasma instead of serum as test material. The samples of blood recipients in our hospital were screened for irregular antibody using pooled test cells. Screening of the antibodies was identified both by MGIAT and CIAT combined papain technique respectively. The results showed that the irregular erythrocyte antibodies were detected in 20 cases from 5 000 recipients screened by MGIAT, using pooled cells, the positive rate was 0.4%. The specificity of 20 cases of irregular antibodies was as follows: 2 cases of anti D, 8 cases of anti E, 1 cases of anti C, 2 cases of anti c, 2 cases of anti Mi a, 2 cases of anti Jk a, 1 case of anti Le a and 2 cases of anti Fy a. Antibody was detected from 19 cases using CIAT. Anti Le a was detected with adding complement from Le(a b ) person. Only 13 cases antibody were found by papain technique. It was concluded that irregular antibody screening by MGIAT using pooled cells can take place of the CIAT combining with papain technique in clinical application. Plasma is superior to serum in antibody screening test.

关 键 词：微柱凝胶间接抗球蛋白法 筛选 鉴定 不规则抗体 抗球蛋白法 

分 类 号：R446.62[医药卫生—诊断学] R457.1[医药卫生—临床医学]