槲皮素体外逆转白血病细胞耐药性的研究  

Study of quercatin reverse druy-resistance of leukemia cell in vitro

作　　者：乔新惠[1] 唐惠芳[2] 宋岚[1] 龙石银[1] 周弟先 刘四春[1] 

机构地区：[1]南华大学基础医学院生化教研室 [2]南华大学基础医学院第一附属医院心内科

出　　处：《中国临床药理学与治疗学》2003年第3期302-303,共2页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖南省教委资助项目 (№ :5 0 0 XJ 0 0 12 0 )

摘　　要：目的 :探讨槲皮素对白血病细胞多药耐药逆转的影响。方法 :以人白血病细胞系K5 62及耐药细胞系K5 62 A0 2 (耐阿霉素 )为实验对象 ,采用MTT法检测细胞毒性 ,流式细胞仪检测P gp表达水平。结果 :1 0 μmol·L- 1 槲皮素对细胞毒性和P gp的表达无影响 ,2 0、40 μmol·L- 1 槲皮素能明显增强化疗药物对细胞的毒性 ,降低P gp表达的水平。结论 :槲皮素能逆转K5 62 A0AIM: To explore the effect of quercetin on resistance revers al and its mechanism. METHODS: Human erythroleukemic cell line K56 2 and its doxorubicin-resistant counterpart K562/A02 were used in the study. Cy totoxicity was assessed by MTT assay. P-gp expression was measured with flow cy tometry. RESULTS: Quercetin of 10 μmol·L -1 had no effect on the multidrug resistance reversal and P-gp expression. However, quercetin o f 20 μmol·L -1 could increase chemosensitivity of F562/A02 and decrease t he P-gp expression. CONCLUSION: Quercetin can reverse drug-res istance of K562/A02 cell to Daunorubicin.

关 键 词：槲皮素 白血病 细胞耐药性 细胞毒性 化疗药物 药效学 

分 类 号：R285.6[医药卫生—中药学] R733.7[医药卫生—中医学]