灵芝多糖对大鼠胰岛细胞分泌胰岛素功能的影响  

Effect of Ganoderma lucidum polysaccharides on the insu lin releasing function of pancreatic islets in rats

作　　者：张慧娜[1] 林志彬[1] 

机构地区：[1]北京大学基础医学院药理学系,北京100083

出　　处：《中国临床药理学与治疗学》2003年第3期265-268,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：上海绿谷 (集团 )有限公司科研基金资助课题

摘　　要：目的 :探讨灵芝多糖 (Gl PS)对胰岛细胞分泌胰岛素的影响。方法 :分离大鼠胰岛细胞 ,分别观察在葡萄糖为 5 .6和 1 6.7mmol·L- 1 时 ,Gl PS对胰岛细胞分泌胰岛素的影响。进一步加入不同的抑制剂观察Gl PS对胰岛细胞分泌胰岛素的影响。采用Westernblotting观察Gl PS对胰岛细胞葡萄糖转运蛋白 2 (GLUT2 )表达的影响。结果 :在 5 .6mmol·L- 1葡萄糖时 ,1 0 0mg·L- 1 的Gl PS可明显促进胰岛细胞胰岛素的分泌 ;当葡萄糖浓度为 1 6.7mmol·L- 1 时 ,5 0和 1 0 0mg·L- 1 的Gl PS均可明显促进胰岛细胞胰岛素的分泌。维拉帕米或维拉帕米 +EGTA均可显著抑制 5 .6和 1 6.7mmol·L- 1 葡萄糖时胰岛素的分泌 ,维拉帕米只能部分抑制Gl PS的促胰岛素分泌作用 ,而维拉帕米 +EGTA则可完全抑制Gl PS的这种作用。在葡萄糖浓度为 5 .6和 1 6.7mmol·L- 1 时 ,Gl PS 5 0和 1 0 0mg·L- 1 均能明显促进胰岛细胞GLUT2的蛋白表达。结论 :Gl PS可能通过促进胰岛细胞GLUT2蛋白的表达从而有助于葡萄糖转运入B细胞 ,促进葡萄糖的代谢 ,引起胰岛细胞外Ca2 +AIM: To investigate the effect of (Ganoderma lucidum ) polysaccharides (Gl-PS) on the insulin-releasing function of pancreati c islets. METHODS: Pancreatic islets were isolated and incubated with 5.6 or 16.7 mmol·L -1 glucose and different concentratio ns of Gl-PS for 1 h. Then the insulin was examined. Verapamil, and verapamil co mbined EGTA were used to testify whether the insulin-releasing effect of Gl-PS was inhibited by these inhibitors. The effects of Gl-PS on protein expression of the glucose transporter 2(GLUT2) under 5.6 and 16.7 mmol·L -1 glucose were also investigated. RESULTS: Gl-PS stimulated th e insulin release when incubated with the glucose of 5.6 or 16.7 mmol ·L -1 . The insulin releasing effect of Gl-PS was partly inhibited by ve rapamil and completely inhibited by verapamil+EGTA. Gl-PS could promote the GLU T2 protein expression of pancreatic islets under glucose of 5.6 and 16.7 mmol·L -1 . CONCLUSION: Gl-PS possesses insulin-rele asing effect under the glucose of 5.6 and 16.7 mmol·L -1 due t o the promotion of the GLUT2 protein expression and the subsequent facilitation of Ca 2+ inflow into the pancreatic B cells.

关 键 词：灵芝多糖 胰岛细胞 胰岛素功能 葡萄糖转运蛋白2 抑制剂 

分 类 号：R285.5[医药卫生—中药学]