分光光度法测定甘油合剂中甘油的含量  

Determination of glycerin content in glycerin mixture by spectrophotometry

作　　者：徐秀丽[1] 成立军[1] 刘伦[1] 

机构地区：[1]泰山医学院第一教学医院,山东泰安271000

出　　处：《泰山医学院学报》2002年第3期247-248,共2页Journal of Taishan Medical College

摘　　要：目的　建立一种甘油合剂中甘油含量测定的方法。方法　根据甘油与氢氧化铜悬浊液作用生成绛蓝色的甘油铜溶液 ,采用分光光度法在 63 0nm波长处测定吸光度 ,建立回归方程 ,以确定甘油的含量。结果　甘油浓度在 4%～ 7%范围内与吸光度呈线性关系 (r=0 9999,P <0 0 1) ,回收率为 10 0 5 % ,RSD为 0 8%。结论　该法简单 ,准确度高 ,精密度好 。Objective: To establish a method for the determination of glycerin content in glycerin mixture. Methods: According to the rational that glycerin reacts with cuprum hydroxide and makes glycerone in dark reddish blue, we measured the absorbance at 630nm by spectrophotometry and established a regression equation to determinate the content of glycerin. Results: The linear range between concentration and absorbance was 4%～7% (γ=0 9999, P<0 01). The recovery rate was 100.5%. RSD was 0.8%. Conclusion: This method is simple, sensitive and accurate, and it is not disturbed by other substances in the mixture.

关 键 词：分光光度法 甘油 含量 测定 

分 类 号：R927.2[医药卫生—药学]