肠血管活性多肽或生长抑素对大鼠肠淋巴细胞在肠淋巴组织分布的影响  

The effect of vasoactive intestinal peptide or somatostatin on the distribution of intestinal lymphocytes in intestinal lymphoid tissue in rats

作　　者：杨辉[1] 唐承薇[1] 

机构地区：[1]四川大学华西医院消化内科,成都610041

出　　处：《中华微生物学和免疫学杂志》2003年第3期192-194,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家杰出青年科学基金资助项目 ( 3972 5 0 12 )

摘　　要：目的　观察肠血管活性多肽 (VIP)或生长抑素 (SST)对大鼠肠淋巴细胞归巢至肠相关淋巴组织 (GALT)的影响。方法　从肠系膜淋巴管插管引流淋巴液 ,淋巴细胞经VIP和SST体外孵育后 ,用51 Cr标记 ,将51 Cr 肠淋巴细胞从股静脉回输入大鼠体内。取出各组织、器官 ,用γ计数器检测其放射性活度。结果　生理状况下 ,约 10 % 51 Cr 肠淋巴细胞在短期内归巢至GALT。经VIP或SST孵育的51 Cr 肠淋巴细胞回输入大鼠体内后 ,在肠系膜淋巴结 ( 1.85 % ,1.6 0 % )和Peyer结 ( 1.83% ,1.5 6 % )分布的百分比显著低于对照组 ( 3.83% ,3.85 % ) ,P <0 .0 5。 2种多肽对51 Cr 肠淋巴细胞在小肠弥散淋巴组织的分布无明显影响。结论　VIP或SST对大鼠肠淋巴细胞归巢至Peyer结和肠系膜淋巴结有一定的抑制作用。Objective To observe the effect of VIP (Vasoactive intestinal peptide) or SST (Somatostatin) on the lymphocyte homing to the intestinal tract. Methods Intestinal lymphocytes were collected from mesenteric lymphatic duct of rats. Intestinal lymphocytes, which had been incubated with VIP or SST, were labeled with 51 Cr and then infused into blood circulation of rats. 51 Cr-intestinal lymphocytes in organs (or tissues) were counted with γ-counter. Results About 10% of 51 Cr-intestinal lymphocytes homed back to intestinal tract within 1?h at normal physiological state. The distribution of 51 Cr-intestinal lymphocytes treated with either VIP or SST in mesenteric node (1.83% or 1.56%) and in Peyer′s patches (1.85% or 1.60%) were significantly less than that in control group (3.83%, 3.85%), P <0.05. VIP or SST did not show remarkable affection on the homing of 51 Cr-intestinal lymphocytes to diffusive lymphatic tissue in small intestine when compared with the control. Conclusion Either VIP or SST reduces the homing of intestinal lymphocytes to mesenteric nodes and Peyer′s patches in rats. [

关 键 词：肠血管活性多肽 生长抑素 肠淋巴组织 归巢 免疫学 

分 类 号：R392[医药卫生—免疫学]