血红蛋白作为过氧化物模拟酶催化测定过氧化氢  

Determination of hydrogen peroxide using hemoglobin as mimetic peroxidase

作　　者：黄应平[1] 刘丰[1] 陈百玲[1] 罗光富[1] 颜克美[1] 李杨[1] 

机构地区：[1]三峡大学化学系,宜昌443000

出　　处：《分析试验室》2003年第1期12-15,共4页Chinese Journal of Analysis Laboratory

基　　金：湖北省教育厅自然科学基金资助项目 (2 0 0 1B140 0 5 )

摘　　要：研究了牛血红蛋白 (Hemoglobin ,Hb)作为过氧化物模拟酶催化H2 O2 氧化隐性亮绿 (RecessiveBrilliantGreen ,RBG)显色反应的催化特性及反应条件。该体系在pH 5 .73的条件下形成的酶催化产物在 640nm处有最大吸收。体系测定H2 O2 时表观摩尔吸光系数为 7.1 5× 1 0 3L·mol-1·cm-1,测定H2 O2 检测限为 2 .8× 1 0 -6mol L。方法可用于天然水中H2 O2 的测定。The catalytic activity of hemoglobin to oxidation of Recessive Brilliant Green (RBG) by peroxide hydrogen was studied. Also, catalytic characteristic and reaction condition of Hb-RBG-H 2O 2 system were discussed. At pH 5.73, products of this system exhibited maximum absorption at 640 nm. The apparent molar absorption for determination of hydrogen peroxide was found to be 7.15×103 L·mol -1·cm -1 with a detection limit of 2.8×10 -6 mol/L. The method was used for determination of trace H 2O 2 with good results.

关 键 词：牛血红蛋白 过氧化物模拟酶 H2O2 分光光度法 

分 类 号：O657.32[理学—分析化学]