尿激酶受体在血栓性疾病中的表达及临床意义  

Study on the Expression of Urokinase-type Plasminogen Activator Receptor in Thrombotic Patients and its Clinical Implications

作　　者：董孝媛[1] 徐从高[1] 孙国瑞[1] 

机构地区：[1]山东大学齐鲁医,济南250012

出　　处：《血栓与止血学》2003年第1期6-9,共4页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的:检测尿激酶型纤溶酶原激活物受体(u-PAR)在阵发性睡眠性血红蛋白尿(PNH)患者和心肌梗死、脑梗死患者中的表达,并探讨其临床意义。方法:PHN组22例,其它血栓形成组20例,其中心肌梗死10例,脑梗死10例,健康对照组20例。以流式细胞术检测外周血白细胞上u-PAR的表达,ELISA法检测血清su-PAR。结果:健康对照组外周血粒细胞中u-RPAR^+细胞比例皆>90％,其它血栓形成组与健康对照组表达率无显著性差异。PNH患者外周血粒细胞u-PAR^+胞显著降低,与其它两组相比,有显著性差异。其它血栓形成组和健康对照组血清su-PAR水平较低,范围较窄,而PNH组血清su-PAR水平显著升高,且离散度较大,与另两组有显著性差异。PNH伴血栓形成者su-PAR水平显著升高。PNH患者中血栓阳性组与血栓阴性组比较,外周血粒细胞u-PAR表达率明显降低,血清su-PAR水平显著升高,均有显著性差异。PNH患者血清su-PAR与u-PAR^+细胞百分率呈负相关。结论:PNH患者外周血粒细胞u-PAR表达率降低,血清su-PAR水平明显增高,与血栓形成并发症相关。以流式细胞术检测细胞u-PAR并检测血清su-PAR有助于监测PHN患者血栓形成。Objective:To investigate the expression of urokinase-type plasrninogen activator receptor (u-PAR) on the peripheral blood granulocytes and soluble u-PAR(su-PAR) ,myocardial infarction and cerebral infarction, and its clinical implications. Methods: A total of 22 PNH patients, 20 other thrombotic patients, including 10 petients with myocerdial infarction and 10 pctiants with carebrel to infarction, and 20 healthy controls. The expression of u-PAR on peripheral blood granulocytes were analyzed with flow cytometry. Soluble u-PAR (su-PAR) in serum was assayed by enzyme-linked immunoadsorbent assay (ELISA) . Results: All the fraction of u-PAR+ granulocytes in the peripheral blood from 20 healthy controls were more than 90% . No remarkable difference was found between the expressions of u-PAR in thrombotic patients and healthy controls. The expression of u-PAR in 22 PNH patients was significantly decreased and the level of serum su-PAR was increased with a greater variation, especially in the patients with thrombosis. In the 22 PNH patients, the expression of u-PRA was significantly decreased and the serum level of su-PAR was significantly elevated in thrombotic patients compared with nonthrombotic patients. The serum level of su-PAR was negatively correlated to the percentage of u-PAR+ granulocytes in PNH patients. Conclusion: In PNH patients, the expression of u-PAR on granulocytes was remarkably decreased and the serum level of su-PAR was increased significantly. Flow cytometric analysis of u-PAR on the peripheral blood granulocytes and detection of su-PAR were of value in monitoring thrombosis in PNH patients.

关 键 词：尿激酶受体 血栓性疾病 表达 临床意义 阵发性睡眠性血红蛋白尿 心肌梗死 

分 类 号：R556.64[医药卫生—血液循环系统疾病] R542.22[医药卫生—内科学]