HPLC测定己酮可可碱氯化钠注射液  

Quantitation of the theobromine injection by high-performance liquid chromatography

作　　者：刘晓鸿[1] 张明生[2] 

机构地区：[1]合肥市第一人民医院药剂科,合肥230061 [2]安徽中医学院第一附属医院药剂科,合肥230031

出　　处：《安徽医药》2003年第1期42-43,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的　建立HPLC法测定己酮可可碱氯化钠注射液中己酮可可碱含量的方法。方法　用十八烷基硅烷键合硅胶柱 (4 6× 15 0mm) ;以甲醇 -水 -冰醋酸 (4 5∶ 5 5∶5 )为流动相 ;检测波长为 2 74nm。结果　线性回归方程为 y =- 10 2 80 [phone]x ,r=0 9999,平均回收率为 10 0 4 0 %。结论　该法简便、准确、快捷 。AIM To develop a high performance liquid chromatography (HPLC) method for determing the injection of theobromine. METHODS Matrine can be separated on symmetry shield column (C 18 5 μm 4 6×150 mm). The mobile phase was methanol∶water∶acetic acid glacial(45∶55∶5 V∶V∶V) selecting 274 nm wavelength. RESULTS The line regressive equation y=-10280+392975 21x, r= 0 9999. The average recovery was 100 40%. CONCLUSIONS The method is simple, quick and accurate. It can be used as an quality control method of the injection.

关 键 词：HPLC 含量测定 己酮可可碱氯化钠注射液 质量控制 

分 类 号：R927.2[医药卫生—药学] R973.2