IL-2对抗精子抗体阳性大鼠附睾精子凋亡的影响及机制探讨  

Effect of Interleukin-2 on sperm apoptosis in antisperm antibody positive rat

作　　者：温海霞[1] 张玮[1] 张大勇[1] 边淑玲[1] 张辉[1] 

机构地区：[1]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2003年第2期107-109,共3页Journal of Harbin Medical University

基　　金：黑龙江省攻关课题 (GC0 1C14 2 -0 1)

摘　　要：目的　探讨IL 2对抗精子抗体 (AsAb)阳性大鼠精子凋亡的影响。方法　建立AsAb阳性大鼠动物模型 ,采用 3′ 原位末端DNA标记 (TUNEL)技术检测大鼠附睾精子凋亡率 ;应用化学比色法测定精子匀浆的总抗氧化能力。结果　AsAb阳性组大鼠精子凋亡率明显高于正常组 (P <0 .0 0 1) ;AsAb +IL 2给药组大鼠精子凋亡率与AsAb阳性组相比显著增高 (P <0 .0 0 1) ;AsAb阳性组大鼠精子匀浆总抗氧化能力与正常组比较显著降低 (P <0 .0 0 1) ;IL 2使AsAb阳性大鼠精子的总抗氧化能力进一步降低 (P <0 .0 0 1)。结论　抗精子抗体阳性大鼠精子凋亡率增加 ;IL 2显著促进精子凋亡 。Objective To investigate the effect of IL 2 on sperm apoptosis in the antisperm antibody(AsAb) positive rat.Methods Animal model of the AsAb positive rat was established. Percentages of apoptotic sperm(PAS) in rat were tested by in situ 3′ end DNA labeing(TUNEL). Changes of total antioxidant capacity of sperm were detected by chemical colorimetry. Results IL 2 could increase PAS of sperm in AsAb positive rat( P <0.001), and decrease further total antioxidant capacity in AsAb positive rat sperm( P <0.001).Conclusion The PAS is increased in AsAb positive rat. IL 2 could increase sperm apoptosis. Increasing oxygen free radicals of sperm might be related to sperm apoptosis.

关 键 词：HL—2 抗精子抗体阳性 大鼠 附睾 精子 凋亡 影响 氧自由基 

分 类 号：R392.11[医药卫生—免疫学]