高效毛细管电泳法同时测定红花中腺苷、芦丁和槲皮素的含量  

Determination of adenosine,rutin and quercetin in Carthamus tinctorius by HPCE

作　　者：孙沂[1] 郭涛[2] 隋因[2] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳军区总医院药剂科,辽宁沈阳110015

出　　处：《药学学报》2003年第4期283-285,共3页Acta Pharmaceutica Sinica

摘　　要：目的　用高效毛细管电泳法对红花中的有效成分腺苷、槲皮素和芦丁同时进行含量测定。方法　采用熔融石英毛细管柱 (66 5cm× 5 0 μmID ,有效长度 5 8cm)作为分离通道 ;以 5 0mmol·L- 1 硼砂其中含 18%甲醇的溶液 (pH9 7)为运行缓冲液 ;运行电压 :2 4kV ;毛细管柱温 :2 0℃ ;检测波长 :2 10nm。结果　以利福平为内标 ,腺苷、芦丁和槲皮素分别在 10～ 160mg·L- 1 ,10 0～ 2 0 0 0mg·L- 1 和 10 0～ 160 0mg·L- 1 线性关系良好 (r>0 998) ;平均回收率分别为98 5 %～ 10 0 5 % ,96 9%～ 99 5 %和 99 1%～ 99 5 % ;RSD分别为 6 5 % ,5 2 %和 5 6% (n =5 )。结论　此法操作简便快速 ,回收率及重现性好 ,可作为红花质量控制的方法。Aim To develop a method for determination of adenosine, rutin and quercetin in Carthamus tinctorius L. by high performance capillary electrophoresis(HPCE). Methods A fused silica capillary (66 5 cm×50 μm ID, an effective length of 58 cm) was used. The running buffer composed of 50 mmol·L -1 borax (pH 9 7) containing 18% methanol. The applied voltage was 24 kV and the capillary temperature was 20 ℃ . The detection wavelength was 210 nm. Rifampicin was used as internal standard. Results A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 10-160 mg·L -1 for adenosine, 100- 2 000 mg·L -1 for rutin and 100- 1 600 mg·L -1 for quercetin ( r >0 998). The average recoveries were 98 5%-100 5%, 96 9%-99 5% and 99 1%-99 5% for adenosine, rutin and quercetin, respectively. The relative standard deviation was less than 6 5%( n =5). Conclusion The method is simple, rapid and with satisfactory recoveries and good reproducibilities. It can be used to control the quality of Carthamus tinctorius .

关 键 词：高效毛细管电泳法 测定 红花 腺苷 芦丁 槲皮 

分 类 号：R284.1[医药卫生—中药学]