高效液相色谱法测定中药石韦2种成分的含量  

Determination of chlorogenic acid and eriodictyol-7-O-β-D-glucuronide in Pyrrosia by RP-HPLC

作　　者：马辰[1] 周圆[2] 刘爱茹[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京100050 [2]沈阳药科大学,辽宁沈阳110021

出　　处：《药学学报》2003年第4期286-289,共4页Acta Pharmaceutica Sinica

基　　金：国家重点科技项目 (攻关 )计划资助项目 (99 92 9 0 1 2 6)

摘　　要：目的　对中药石韦中的 2种成分绿原酸和圣草酚 7 O β D 吡喃葡糖醛酸苷的分析方法进行研究 ,并测定了不同种、不同产地石韦中 2种成分的含量。方法　用反相C1 8柱 ,甲醇 水 磷酸 (5 0∶2 0 0∶0 2 )为流动相 ,2 84nm作为检测波长。结果　绿原酸和圣草酚 7 O β D 吡喃葡糖醛酸苷的含量分别在 0 0 1～ 5 μg和 0 0 0 4～ 5 μg有良好的线性关系 (r=0 9997) ,样品的回收率分别为 97 9% (RSD =2 9% )和 99 1% (RSD =2 4% )。结论　采用RP HPLC法对 2 1个不同种、不同产地石韦样品进行了测定 ,不同产地、不同种石韦中绿原酸及圣草酚 7 O β D 吡喃葡糖醛酸苷的含量相差悬殊 。Aim To develop a method for determination of chlorogenic acid and eriodictyol 7 O β D glucuronide in Pyrrosia of different species and different places of origin by RP HPLC. Methods Chromatography was performed using a C 18 column with mobile phase of methanol water phosphoric acid (50∶200∶0 2). The monitoring wavelength was 284 nm. Results The linear ranges were 0 01-5 0 μg ( r =0 999 7) and 0 004-5 0 μg ( r =0 999 7), the recoveries were 97 1% ( n =8, RSD=2 7%) and 98 8% ( n =9, RSD=2 5%) for chlorogenic acid and eriodictyol 7 O β D glucuronide, respectively. Conclusion The method was employed to the analysis of 21 samples of Pyrrosia . The contents of compounds vary greatly depending on the species used, place of collection and time of harvesting. The HPLC method is sensitive, rapid and can be used to control the quality of Pyrrosia and to guide reasonable season of harvesting.

关 键 词：高效液相色谱法 测定 中药 石韦 含量 

分 类 号：R284.1[医药卫生—中药学]