高温高湿环境下热习服犬肢体火器伤后SOD、MDA含量变化的实验研究  

EXPERIMENTAL STUDY ON THE EFFECT OF HEAT ACCLIMATIZATION ON THE CONTENT OF SOD AND MDA IN DOG WITH GUNSHOT WOUNDS IN LIMBS IN HOT AND HUMID ENVIRONMENT

作　　者：张旭辉[1] 裴国献[1] 魏宽海[1] 周祥吉[2] 孙鸿涛[1] 胡罢生[1] 

机构地区：[1]第一军医大学南方医院全军创伤骨科中心 [2]解放军第89医院

出　　处：《解放军医学杂志》2003年第4期293-294,共2页Medical Journal of Chinese People's Liberation Army

基　　金：全军"九五"医药卫生杰出中青年基金 (编号 98J0 0 4 );全军医学科研"十五"计划重点课题 (编号 0 2Z0 0 1 )资助课题

摘　　要：为研究高温高湿环境下热习服犬肢体火器伤后超氧化物歧化酶 (SOD)、丙二醛 (MDA)含量变化情况 ,将 18只犬随机分为热习服组、常温常湿组、高温高湿组 ,分别在火器致伤前、后不同时间点检测伤道肌组织中SOD、MDA含量。结果发现 ,热习服组和高温高湿组中MDA含量均在伤后 1h开始升高 ,其中前者升高幅度明显低于后者 ,前者达峰值时间为 10h ,较后者推迟 4h且峰值减低 (P <0 0 5 ) ,18h后三组MDA含量无明显差异 ;热习服组SOD含量在伤后即开始上升 ,较其他两组早 1h,且其 4h以后的SOD值高于高温高湿组 ;高温高湿组在 10h时SOD值降至测不出结果 ,比其他两组早 4h。以上结果说明 ,SOD、MDA参与了高温高湿环境下肢体火器伤后机体的病理生理过程 ;热习服能在一定程度上缓解伤后机体的病理变化 ,可作为高温高湿环境下防治肢体战创伤的有效手段。To explore the effect of heat acclimatization to the content of SOD and MDA in dogs with gunshot wound in limbs in hot and humid environment, eighteen Chinese mongrels were divided into 3 groups randomly according to the given environment: heat acclimatization(HA) group, normal environment(NE)group, and hot and humid environment(HHE) group. The contents of superoxide dismutast(SOD) and malonaldehyde(MDA) in muscle tissues of the gunshot wound track were assayed at 0, 1, 3, 4, 6, 8, 10, 14, 18 an 24h respectively after wounding. The results showed that the contents of MDA in both HA and HHE groups began to ascend on postinjury hour(PIH) 1, peaking at PIH10 in HA group and PIH6 in HHE group, with the former lower than the latter. There was no significant difference between the contents of MDA in the three groups at PIH18. The content of SOD in HA group began to rise immediately after injury, which was 1h earlier than that in the other two groups, and it was higher than that in HHE group after PIH4. The content of SOD was too low to be measured at PIH10 in HHE group, and this result was 4h earlier than that in the other two groups. In conclusion, SOD and MDA play an important role in the pathophysiological process of gunshot wound in hot and humid environment. Heat acclimatization can be favorable to mitigate pathophysiological changes, and it might be an effective method to prevent rapid deterioration of gunshot injuries in hot and humid environment.

关 键 词：高温高湿环境 热习服 犬 肢体火器伤 SOD MDA 含量 实验研究 

分 类 号：R826.5[医药卫生—临床医学]