急性辐射损伤小鼠T细胞功能亚群的变化  

CHANGES IN T CELL SUBSETS OF MICE AFTER ACUTE IRRADIATION INJURY

作　　者：赵莲[1] 董波[1] 熊国林[1] 赵蓓蕾[1] 罗庆良[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《解放军医学杂志》2003年第4期332-334,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军"九五"指令基金资助课题 (编号 960 50 0 1 )

摘　　要：为了解T细胞功能亚群在急性辐射损伤后的改变 ,探讨急性放射病细胞免疫功能长期低下的发病机制 ,用60 Coγ射线 5Gy对二级Balb/c小鼠一次性全身照射。在照射后约 10、2 8和 12 0天用双标记免疫荧光流式细胞术检测脾脏淋巴细胞CD4 + 和CD8+ T细胞亚群 ,用相对定量的RT PCR法检测脾脏细胞经ConA刺激活化后IL 4基因表达情况。结果发现 ,照射组相对于对照组CD4 + /CD8+ 比值显著升高 ,随着照射后时间的延长 ,CD4 + /CD8+ 比值逐渐降低 ,但至 12 0天左右仍未恢复到同批正常对照的水平。受照后 10天左右的小鼠脾细胞IL 4基因在mRNA水平明显升高。提示小鼠CD8+ T细胞比CD4 + T细胞辐射敏感性强 。In order to evaluate the changes in T cell subsets after acute irradiation injury, and investigate the mechanism of deficiency of cellular immunity in acute irradiation syndrome, Balb/c mice were exposed to a single total body irradiation of 5Gy gamma rays from a 60 Co source. On days 10, 28 and 120, the amounts of CD4 + and CD8 + subsets in spleen cells were evaluated by flow cytometry (FCM), and expression of IL 4 gene in ConA stimulated spleen cells was evaluated by reverse transcription linked polymerase chain reaction, in which β-actin gene was used as an internal control. It was found that after irradiation, the ratio of CD4 + /CD8 + became significantly higher than that of the controls, and then the ratio lowered gradually until day 120. However, it still did not return to the level of the controls. On day 10, expression of IL 4 gene at mRNA level increased significantly. The results indicated that CD8 + subset was more radiosensitive than CD4 + , and the pattern of cytokine seeretion shifted to that of Th2 soon after irradiation.

关 键 词：急性辐射损伤 小鼠 T细胞功能亚群 发病机制 细胞免疫功能 

分 类 号：R818[医药卫生—放射医学]