中国汉族人SDF1-β编码区新多态性位点初步研究  

RESEARCH ON THE POLYMORPHISM PROPERTY OF CXCR4 LIGAND SDF1-β CODING REGION IN CHINESE HAN NATIONALITY

作　　者：刘明旭[1] 王福生[1] 洪卫国[1] 王波[1] 金磊[1] 雷周云[1] 侯静[1] 

机构地区：[1]解放军第302医院

出　　处：《解放军医学杂志》2003年第4期305-306,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39770 6830 )

摘　　要：为调查中国汉族人HIV 1相关基因SDF 1β的多态性特点 ,选择 4 5例健康汉族人 ,对SDF 1β编码区的 4个外显子进行PCR扩增 ,然后分别测序。用DNAstar软件分析测序结果 ,在编码区共发现 1个基因多态性 (SNP)位点 :192位G→T ,使赖氨酸变为天冬酰胺 ,突变频率为 8 9% ;1例单碱基缺失 :10 0位T缺失 (10 0ΔT) ,引起 34位氨基酸移码突变 ,到 5 9位氨基酸翻译提前终止。两者均为首次发现 ,其对HIVForty five Han individuals were enrolled for the investigation of the polymorphism property of HIV 1 associated SDF 1β in Chinese Han nationality. Four exons of SDF 1β were cloned by PCR amplification using 4 pairs of primers, then sequenced respectively. DNAstar software was employed to analyze the sequencing results. One SNP and one thymidine deletion were identified in the coding region of SDF1 β. The SNP was G→T at 192 with a frequency of 8.9%, resulting in replacing lysine to asparagine. The thymidine deletion was at 100 (100ΔT), which caused frame shift from the 34th amino acid and ending at the 59th amino acid. Both of them were discovered for the first time, and their influences on HIV 1 infection and AIDS progression remain to be explored.

关 键 词：中国 汉族人 SDF1—β编码区 新多态性位点 研究 

分 类 号：Q987[生物学—遗传学]