商陆DNA导入水稻引起后代性状变异初报  

Variation of rice progeny derivws from introduction of DNA from Phytolaoca esculenta

作　　者：文锦芬[1] 邓明华[2] 李良勇[3] 萧浪涛[1] 彭克勤[1] 洪亚辉[1] 胡家金[1] 

机构地区：[1]湖南农业大学理学院,湖南长沙410128 [2]湖南农业大学园艺园林学院,湖南长沙410128 [3]湖南农业大学农学院,湖南长沙410128

出　　处：《作物研究》2003年第1期13-15,共3页Crop Research

基　　金：湖南省计委资助(94-352-16)。

摘　　要：将商陆DNA通过浸种法、花粉管导入法和穗茎注射法处理水稻,比较3种导入方法对当代结实率和变异率的影响以及导入后代的遗传变异情况。结果表明:花粉管导入法对导入当代结实率和后代变异率影响均最大,商陆DNA处理水稻后引起了水稻若干数量性状和质量性状的较大遗传变异。The DNA from Phytolaoca esculenta Van houtt was introduced into rice by seed soaking,pollen tube pathway technique or spikestalk microinjection.The 3 procedures of DNA introduction were compared in respect to effects on seed set and variation of the recipients and their progeny.The results showed that pollen tube pathway technique was the most efficient in that it brought about the greatest variation,quantitative or qualitative,of rice ricipients and their progeny.

关 键 词：水稻 商陆DNA 转基因 浸种法 花粉管导入法 穗茎注射法 当代结实率 后代变异率 性状变异 

分 类 号：S511[农业科学—作物学]