玉米细胞悬浮系的建立与单细胞培养效果  

Establishment of Suspension Cell Line and Monocell Culture of Maize(Zea mays L.)

作　　者：马莲菊[1] 高峰[2] 于翠梅[1] 刘世强[1] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110161 [2]沈阳市农村经济发展局,辽宁沈阳110013

出　　处：《沈阳农业大学学报》2002年第6期449-451,共3页Journal of Shenyang Agricultural University

基　　金：辽宁省科委"九五"自然科学基金项目

摘　　要：以330、辽7352和丹340共3个玉米自交系的雄幼穗为外植体获取愈伤组织,建立悬浮细胞系,在改良MS、改良B5、改良1/2B5 培养基中进行细胞悬浮培养。试验中发现 ,基因型和培养基对细胞悬浮系有很大影响,其中供试材料330表现最好,圆形细胞率平均达16.5%,改良1/2B5 培养基有利于细胞悬浮培养,500mg·L-1的水解酪蛋白对悬浮细胞生长有促进作用。2,4 -D的浓度影响悬浮细胞的生长和细胞成活率,浓度为1.0～2.0mg·L-1时比较适宜。细胞悬浮培养5～7d继代1次。在悬浮培养1个月后 ,经400目镍网过滤筛选,获得单细胞。单细胞培养中,激素水平和培养方式影响单细胞的分裂率,其中2,4-D的浓度为0.5～1.0mg·L-1时比较适宜。浅层培养和看护培养有利于单细胞的生长。在单细胞培养过程中试验获得了愈伤组织。Young male panicles from3inbred of Zea mays L.were used as explants to induce calli.Calli were used to establish suspension cell lines.Cell suspension cultures were made on improved MS,B 5 and1/2B 5 media.The experiment results indicated that genotypes and media had an effect on suspension cell line.The best genotype is inbred330,whose circular cell rate reached16.5percent on average.Improved1/2B 5 media was optimal for cell suspension culture.500mg·L -1 casein hydrolysate could proˉmote the growth and living of suspension cells.Different2,4-D concentrations influenced the growth and active rate of suspension cells.1.0-2.0mg·L -1 2,4-D was optimal.Suspension cells were in mid-log phase by5-7days.Monocells filtered with a400#nickel screen were acquired after one month.Genotypes and hormone concentrations influenced monocell division frequencies,0.5-1.0mg·L -1 2,4-D was optimal.Shallow layer culture and nurse culture were optimal for monocell growth.The calli were obtained during monocell culture.

关 键 词：玉米 细胞悬浮系 单细胞培养 愈伤组织 培养基 基因型 

分 类 号：S513[农业科学—作物学]